| County |
Location |
Mile[2] |
Intersects |
Notes |
 begins as a secondary route |
| Wayne |
Waynesboro |
0.0 |
  US-64 / SR-15 (unsigned) (Dexter L. Woods Memorial Boulevard) |
|
| Lewis |
Hohenwald |
| 28.5 |
 SR-20 (East Fourth Avenue) |
SR-20 concurrent for 0.7 mi |
| 28.8 |
 SR-48 / SR-20 (South Park Street) |
SR-48 concurrent with SR-99 and SR-20 for 0.4 mi |
| 29.2 |
 US-412 / SR-20 / SR-48 (Main Street) |
concurrency with SR-48 and SR-20 end |
US-412 concurrent for 41.8 mi and primary route  is unsigned |
| 37.4 |
 Natchez Trace Parkway |
|
| Maury |
|
47.0 |
 SR-166 (Pisgah Ridge Road) |
|
| Columbia |
| 57.2 |
  US-43 / US-412 / SR-6 (Columbia Bypass) |
SR-99 continues into downtown Columbia concurrent with US 412 BUS |
| 59.3 |
 SR-50 west (Williamsport Pike) |
SR-50 concurrent with SR-99 and US 412 BUS for 1.1 mi |
| 60.4 |
SR-50 east (James Campbell Boulevard) |
concurrency with SR-50 ends (US 412 BUS still concurrent) |
| 61.4 |
 SR-243 south (Trotwood Avenue) |
|
| 62.0 |
  US-31 / SR-7 (unsigned) (North Garden Street) |
US 31 concurrent for 1.9 mi and SR-7 concurrent for 0.5 mi |
| 62.5 |
SR-7 north (North James Campbell Boulevard) |
concurrency with SR-7 ends and concurrency with US 31 and US 412 BUS continues |
| 63.9 |
US-31 / US-43 / US-412 / SR-6 (unsigned) (Bear Creek Pike) |
US 412 BUS ends and SR-99 rejoins concurrency with main US 412 route/US 31 concurreny ends |
| 71.0 |
 I-65 |
|
| eastern national terminus of US-412 , SR-99 signage reusmes |
|
74.9 |
  US-431 / SR-106 (unsigned) (Lewisburg and Franklin Pike) |
US 431 and SR-106 concurrent for 0.8 mi |
|
75.7 |
US-431 / SR-106 (unsigned) (Lewisburg and Franklin Pike) |
US 431 and SR-106 concurrency ends |
| Marshall |
|
79.5 |
 SR-272 (Verona Caney Road) |
|
| Chapel Hill |
| 83.1 |
  US-31A / SR-11 (unsigned) (Sam Davis Higway) |
US 31A and SR-11 concurrent for 3.3 mi |
| reverts to a secondary route |
| 83.9 |
 SR-270 (Old Columbia Road) |
|
| 86.4 |
US-31A / SR-11 (unsigned) (Nashville Highway) |
US 31A and SR-11 concurrency ends |
| Rutherford |
Eagleville |
| 92.9 |
  US-41A / SR-16 (unsigned) (South Main Street) |
US 41A and SR-16 concurrent for 1.5 mi |
| 94.4 |
 US-41A / SR-269 / SR-16 (unsigned) |
US 41A and SR-16 concurrency ends and SR-269 concurrent for 5.5 mi |
|
99.9 |
SR-269 (Jackson Ridge Road) |
SR-269 concurrency ends |
| Murfreesboro |
| 110.3 |
 I-24 |
interchange currently under construction |
| 111.6 |
 SR-96 (Old Fort Parkway) |
SR-96 concurrent for 0.6 mi and SR-99 is unsigned |
| 112.2 |
     US 70S/US 41/US 231/SR-1(unsigned)/SR-10(unsigned) (NW Broad Street) |
US 70S and SR-1 concurrent for 1.2 mi, US 41 concurrent for 1.3 mi, US 231 and SR-10 concurrent for 0.7 mi, SR-96 concurrency ends |
| 112.9 |
US-231 / SR-10 (unsigned) (South Church Street) |
US 231 and SR-10 concurrency ends, US 70S, US 41, and SR-1 concurrency continues |
| 113.4 |
 US-70S / US-41 / SR-1 (unsigned) / SR-2 (unsigned) (Mercury Boulevard) |
US 70S and SR-1 concurrency ends, US 41 concurrency continues, and SR-2 concurrent for 0.1 mi |
| 113.5 |
US-41 / SR-2 (unsigned) (SE Broad Street) |
US 41 and SR-2 concurrency ends |
| Cannon |
Bradyville |
128.4 |
 SR-64 (Bradyville Road) |
|
| ends as a secondary route |
