Taxadiene synthase

From Wikipedia, the free encyclopedia

In enzymology, a taxadiene synthase (EC 4.2.3.17) is an enzyme that catalyzes the chemical reaction

geranylgeranyl diphosphate taxa-4,11-diene + diphosphate

Hence, this enzyme has one substrate, geranylgeranyl diphosphate, and two products, taxa-4,11-diene and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, taxa-4,11-diene-forming). Other names in common use include geranylgeranyl-diphosphate diphosphate-lyase (cyclizing,, and taxadiene-forming). This enzyme participates in diterpenoid biosynthesis.

[edit] References

• IUBMB entry for 4.2.3.17
• BRENDA references for 4.2.3.17 (Recommended.)
• PubMed references for 4.2.3.17
• PubMed Central references for 4.2.3.17
• Google Scholar references for 4.2.3.17
• Croteau R (1995). "Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew". J. Biol. Chem. 270: 8686–90. PMID 7721772. 
• Hezari M, Lewis NG, Croteau R (1995). "Purification and characterization of taxa-4(5),11(12)-diene synthase from Pacific yew (Taxus brevifolia) that catalyzes the first committed step of taxol biosynthesis". Arch. Biochem. Biophys. 322: 437–44. PMID 7574719. 
• Lin X, Hezari M, Koepp AE, Floss HG, Croteau R (1996). "Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in Pacific yew". Biochemistry. 35: 2968–77. PMID 8608134. 
• Hezari M, Ketchum RE, Gibson DM, Croteau R (1997). "Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures". Arch. Biochem. Biophys. 337: 185–90. PMID 9016812. 
• Coates RM, Williams RM, Croteau R (2000). "Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase". Chem. Biol. 7: 969–77. PMID 11137819. 

[edit] External links

The CAS registry number for this enzyme class is 169277-52-5.

• IUBMB entry for 4.2.3.17

• KEGG entry for 4.2.3.17

• BRENDA entry for 4.2.3.17

• NiceZyme view of 4.2.3.17

• EC2PDB: PDB structures for 4.2.3.17

• PRIAM entry for 4.2.3.17

• PUMA2 entry for 4.2.3.17

• IntEnz: Integrated Enzyme entry for 4.2.3.17

• MetaCyc entry for 4.2.3.17

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0050553: taxadiene synthase

• AMIGO entry for GO code 0050553: taxadiene synthase