Strictosidine synthase

From Wikipedia, the free encyclopedia

In enzymology, a strictosidine synthase (EC 4.3.3.2) is an enzyme that catalyzes the chemical reaction

3-alpha(S)-strictosidine + H₂O $\rightleftharpoons$ tryptamine + secologanin

Thus, the two substrates of this enzyme are 3-alpha(S)-strictosidine and H₂O, whereas its two products are tryptamine and secologanin.

This enzyme belongs to the family of lyases, specifically amine lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is 3-alpha(S)-strictosidine tryptamine-lyase (secologanin-forming). Other names in common use include strictosidine synthetase, STR, and 3-alpha(S)-strictosidine tryptamine-lyase. This enzyme participates in terpenoid biosynthesis and indole and ipecac alkaloid biosynthesis.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2FP8, 2FP9, 2FPB, and 2FPC.

[edit] References

IUBMB entry for 4.3.3.2
BRENDA references for 4.3.3.2 (Recommended.)
PubMed references for 4.3.3.2
PubMed Central references for 4.3.3.2
Google Scholar references for 4.3.3.2
Treimer JF, Zenk MH (1979). "Purification and properties of strictosidine synthase, the key enzyme in indole alkaloid formation". Eur. J. Biochem. 101: 225–33. doi:10.1111/j.1432-1033.1979.tb04235.x. PMID 510306.
Kutchan TM (1993). "Strictosidine: from alkaloid to enzyme to gene". Phytochemistry. 32: 493–506. doi:10.1016/S0031-9422(00)95128-8. PMID 7763429.
de Waal A, Meijer AH, Verpoorte R (Pt 2). "Strictosidine synthase from Catharanthus roseus: purification and characterization of multiple forms". Biochem. J. 306: 571–80. PMID 7887913.
Ruppert M, Woll J, Giritch A, Genady E, Ma X, Stockigt J (2005). "Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system". Planta. 222: 888–98. doi:10.1007/s00425-005-0031-0. PMID 16133216.
McCoy E, Galan MC, O'Connor SE (2006). "Substrate specificity of strictosidine synthase". Bioorg. Med. Chem. Lett. 16: 2475–8. doi:10.1016/j.bmcl.2006.01.098. PMID 16481164.
Ma X, Panjikar S, Koepke J, Loris E, Stockigt J (2006). "The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins". Plant. Cell. 18: 907–20. doi:10.1105/tpc.105.038018. PMID 16531499.