Smart ligands
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Smart ligands are affinity ligands selected with pre-defined equilibrium (Kd), kinetic (koff, kon) and thermodynamic (ΔH, ΔS) parameters of biomolecular interaction.
Ligands with desired parameters can be selected from large combinatorial libraries of biopolymers using instrumental separation techniques with well-described kinetic behaviour, such as Kinetic Capillary Electrophoresis (KCE), Surface Plasmon Resonance (SPR) and etc. Known examples of smart ligands include DNA smart aptamers; however, RNA and peptide smart aptamers can also be developed.
Smart ligands can find a set of unique applications in biomedical research, drug discovery and proteomic studies. For example, a panel of DNA smart aptamers has been recently used to develop affinity analysis of proteins with ultra-wide dynamic range of measured concentrations.
[edit] References
- Drabovich, A.; Berezovski, M.; Krylov, S.N. Selection of smart aptamers by equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM). J. Am. Chem. Soc. 2005, 127, 11224-11225. PMID: 16089434
- Drabovich, A.P. ; Berezovski, M.; Okhonin, V.; Krylov, S.N. Selection of smart aptamers by methods of kinetic capillary electrophoresis. Anal. Chem. 2006, 78, 3171-3178. PMID: 16643010
- Drabovich, A.P. ; Okhonin, V.; Berezovski, M.; Krylov, S.N. Smart aptamers facilitate multi-probe affinity analysis of proteins with ultra-wide dynamic range of measured concentrations. J. Am. Chem. Soc. 2007, 129, 7260-7261. PMID: 17503828
- Krylov, S.N. Kinetic CE: foundation for homogeneous kinetic affinity methods. Electrophoresis 2007, 28, 69-88. PMID: 17245689
