Small nucleolar RNA SNORD95

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Small nucleolar RNA SNORD95

Type:	Gene; snRNA; guide; CD-box;
2° structure:	Predicted; PFOLD
Seed alignment:	PubMed
Avg length:	68.3 nucleotides
Avg identity:	80%

snoRNA U95 (also known as SNORD95 or Z38) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U95 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs^[1].

U95 was identified by computational screening of the introns of ribosomal protein genes for conserved C/D box sequence motifs and expression experimentally verfied by northern blotting ^[2]. U95 is predicted to guide the 2'O-ribose methylation of 28S ribosomal RNA (rRNA) residues A2802 and C2811 ^[2]^[3].

[edit] References

^ Galardi, S; Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I (2002). "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro". Mol Cell Biol 22: 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMID 12215523.
^ ^a ^b Vitali P, Royo H, Seitz H, Bachellerie JP, Hüttenhofer A, Cavaillé J (2003). "Identification of 13 novel human modification guide RNAs". Nucleic Acids Res. 31 (22): 6543–51. PMID 14602913.
^ Lestrade, L; Weber MJ (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Res. 34: D158–D162. doi:10.1093/nar/gkj002. PMID 16381836.