Small nucleolar RNA SNORD82

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Small nucleolar RNA SNORD82

Type:	Gene; snRNA; guide; CD-box;
2° structure:	Predicted; PFOLD
Seed alignment:	Griffiths-Jones SR
Avg length:	69.8 nucleotides
Avg identity:	88%

snoRNA U82 (also known as SNORD82 or Z25) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U82/Z25 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs^[1].

snoRNA U82 has been identified in both humans and mice^[2]. It is located in the the fifth intron of the nucleolin gene in both species. Two additional snoRNAs (C/D box snoRNA U20 and the H/ACA snoRNA U23 ) are also encoded within the introns of the nucleolin gene^[3].

U82 is predicted to guide the 2'O-ribose methylation of 18S ribosomal RNA (rRNA) residue A1678 ^[3].

Another, different snoRNA, named U82 has been predicted in the introns of L3 ribosomal protein gene (RPL3) in humans and cows^[4]. However, the expresion of this snoRNA could not be confirmed by northern blotting or Reverse transcription polymerase chain reaction (RT-PCR) and it should not be confused with this snoRNA located in the nucleolin gene.

[edit] References

^ Galardi, S; Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I (2002). "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro". Mol Cell Biol 22: 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMID 12215523.
^ Rebane A, Metspalu A (1999). "U82, a novel snoRNA identified from the fifth intron of human and mouse nucleolin gene". Biochim. Biophys. Acta 1446 (3): 426–30. PMID 10524220.
^ ^a ^b Lestrade, L; Weber MJ (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Res. 34: D158–D162. doi:10.1093/nar/gkj002. PMID 16381836.
^ Duga S, Asselta R, Malcovati M, Tenchini ML, Ronchi S, Simonic T (2000). "The intron-containing L3 ribosomal protein gene (RPL3): sequence analysis and identification of U43 and of two novel intronic small nucleolar RNAs". Biochim. Biophys. Acta 1490 (3): 225–36. PMID 10684968.