Small nucleolar RNA SNORD47

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Small nucleolar RNA SNORD47

Type:	Gene; snRNA; guide; CD-box;
2° structure:	Predicted; ILM; Moxon SJ, Daub J
Seed alignment:	Moxon SJ
Avg length:	74.9 nucleotides
Avg identity:	88%

SNORD47 (also known as U47) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U57 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs^[1].

This snoRNA was oroginally cloned from HeLa cells and expression verified by northern blotting ^[2]. It is predicted to guide 2'O-ribose methylation of ribosomal RNA (rRNA) 28S at residue C3866. The mouse orthologue was also cloned ^[3]

This snoRNA is encoded in the introns of the same genes as other C/D bx snoRNAs U44, U74, U75, U76, U77, U78, U79, U80 and U81.^[4].

[edit] References

^ Galardi, S; Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I (2002). "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro". Mol Cell Biol 22: 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMID 12215523.
^ Kiss-Laszlo, Z; Henry Y, Bachellerie JP, Caizergues-Ferrer M, Kiss T (1996). "Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs". Cell 85: 1077–1088. doi:10.1016/S0092-8674(00)81308-2. PMID 8674114.
^ Huttenhofer, A; Kiefmann M, Meier-Ewert S, O'Brien J, Lehrach H, Bachellerie JP, Brosius J (2001). "RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse". EMBO J 20: 2943–2953. doi:10.1093/emboj/20.11.2943. PMID 11387227.
^ Lestrade, L; Weber MJ (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Res. 34: D158–D162. doi:10.1093/nar/gkj002. PMID 16381836.