Small nucleolar RNA SNORD45
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snoRNA U45 (also known as SNORD45) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.
snoRNA U45 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs[1].
U45 was originally cloned from HeLa cells and expression verified by northern blotting [2]. It is related to the snoRNA (MBII-401) identified in mouse [3]
In the human genome there are three very closely related copies of snoRNA U45 (called U45A, U45B, U45C) located within the introns of the same host gene[4].
[edit] References
- ^ Galardi, S; Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I (2002). "Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro". Mol Cell Biol 22: 6663–6668. doi: . PMID 12215523.
- ^ Kiss-László Z, Henry Y, Bachellerie JP, Caizergues-Ferrer M, Kiss T (1996). "Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs". Cell 85 (7): 1077–88. PMID 8674114.
- ^ Huttenhofer, A; Kiefmann M, Meier-Ewert S, O'Brien J, Lehrach H, Bachellerie JP, Brosius J (2001). "RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse". EMBO J. 20: 2943–2953. doi: . PMID 11387227.
- ^ Lestrade, L; Weber MJ (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Res. 34: D158–D162. doi: . PMID 16381836.