Small Cajal body specific RNA 21

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Small Cajal body specific RNA 21

Type:	Gene;snRNA;snoRNA;scaRNA;
2° structure:	PMID:16373490
Seed alignment:	Predicted; RNAalifold; Daub J
Avg length:	139.0 nucleotides
Avg identity:	76%

In molecular biology, Small Cajal body specific RNA 21 (also known as scaRNA21 or ACA68) is a small nucleolar RNA found in Cajal bodies and believed to be involved in the pseudouridylation of U12 minor spliceosomal RNA.

scaRNAs are a specific class of small nucleolar RNAs that localise to the Cajal bodies and guide the modification of RNA polymerase II transcribed spliceosomal RNAs U1, U2, U4, U5 and U12.^[1]

ACA68 (SCARNA21) is a member of the H/ACA box class of snoRNAs that guide the sites of modification of uridines to pseudouridines. This snoRNA was identified by computational screening and its expression in mouse experimentally verified by Northern blot and primer extension analysis ^[2]. ACA68 is proposed to guide the pseudouridylation of residue U19 in U12 snRNA ^[3].

[edit] References

^ Darzacq, X; Jady BE, Verheggen C, Kiss AM, Bertrand E, Kiss T (2002). "Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs". EMBO J. 21: 2746–2756. doi:10.1093/emboj/21.11.2746. PMID 12032087.
^ Schattner P, Barberan-Soler S, Lowe TM; (2006). "A computational screen for mammalian pseudouridylation guide H/ACARNAs.". RNA 12: 15–25. doi:10.1261/rna.2210406. PMID 16373490.
^ Lestrade L, Weber MJ; (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D boxsnoRNAs.". Nucleic Acids Res 34: D158–D162. doi:10.1093/nar/gkj002. PMID 16381836.