SF3B4

From Wikipedia, the free encyclopedia

Splicing factor 3b, subunit 4, 49kDa

PDB rendering based on 1x5t.

Available structures: 1x5t, 1x5u

Identifiers

Symbol(s)

SF3B4; MGC10828; SAP49; SF3b49

External IDs

OMIM: 605593 MGI: 109580 HomoloGene: 22334

Gene ontology
Molecular Function:	• nucleotide binding • nucleic acid binding • protein binding • RNA splicing factor activity, transesterification mechanism
Cellular Component:	• nucleus • spliceosome
Biological Process:	• nuclear mRNA splicing, via spliceosome • RNA splicing

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

n/a

Refseq

NM_005850 (mRNA)
NP_005841 (protein)

XM_001001232 (mRNA)
XP_001001232 (protein)

Location

Chr 1: 148.16 - 148.17 Mb

Chr 3: 96.26 - 96.26 Mb

Pubmed search

[1]

[2]

Splicing factor 3b, subunit 4, 49kDa, also known as SF3B4, is a human gene.^[1]

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.^[1]

[edit] References

^ ^a ^b Entrez Gene: SF3B4 splicing factor 3b, subunit 4, 49kDa.

[edit] Further reading

Champion-Arnaud P, Reed R (1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.". Genes Dev. 8 (16): 1974–83. PMID 7958871.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. PMID 9373149.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Das BK, Xia L, Palandjian L, et al. (2000). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.". Mol. Cell. Biol. 19 (10): 6796–802. PMID 10490618.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell 5 (5): 779–87. PMID 10882114.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMID 11500380.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.". EMBO J. 21 (18): 4978–88. PMID 12234937.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Golas MM, Sander B, Will CL, et al. (2003). "Molecular architecture of the multiprotein splicing factor SF3b.". Science 300 (5621): 980–4. doi:10.1126/science.1084155. PMID 12738865.
Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.". Genomics 83 (1): 153–67. PMID 14667819.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.". RNA 10 (6): 929–41. PMID 15146077.
Nishanian TG, Waldman T (2004). "Interaction of the BMPR-IA tumor suppressor with a developmentally relevant splicing factor.". Biochem. Biophys. Res. Commun. 323 (1): 91–7. doi:10.1016/j.bbrc.2004.08.060. PMID 15351706.
Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo.". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMID 15456888.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Guo D, Han J, Adam BL, et al. (2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.". Biochem. Biophys. Res. Commun. 337 (4): 1308–18. doi:10.1016/j.bbrc.2005.09.191. PMID 16236267.
Terada Y, Yasuda Y (2007). "Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145.". Mol. Cell. Biol. 26 (21): 8149–58. doi:10.1128/MCB.01170-06. PMID 16923959.