SF3B2

From Wikipedia, the free encyclopedia

Splicing factor 3b, subunit 2, 145kDa

PDB rendering based on 2do5.

Available structures: 2do5

Identifiers

Symbol(s)

SF3B2; SAP145; SF3B145; SF3b1; SF3b150

External IDs

OMIM: 605591 MGI: 2441856 HomoloGene: 6678

Gene ontology
Molecular Function:	• nucleic acid binding • protein binding
Cellular Component:	• nucleus • spliceosome
Biological Process:	• nuclear mRNA splicing, via spliceosome • mRNA processing • RNA splicing

Orthologs

Human

Mouse

n/a

Refseq

NM_006842 (mRNA)
NP_006833 (protein)

NM_030109 (mRNA)
NP_084385 (protein)

Location

Chr 11: 65.58 - 65.59 Mb

Chr 19: 5.27 - 5.3 Mb

Pubmed search

[1]

[2]

Splicing factor 3b, subunit 2, 145kDa, also known as SF3B2, is a human gene.^[1]

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.^[1]

[edit] References

^ ^a ^b Entrez Gene: SF3B2 splicing factor 3b, subunit 2, 145kDa.

[edit] Further reading

Agell N, Aligué R, Alemany V, et al. (1998). "New nuclear functions for calmodulin.". Cell Calcium 23 (2-3): 115–21. PMID 9601606.
Champion-Arnaud P, Reed R (1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.". Genes Dev. 8 (16): 1974–83. PMID 7958871.
Gozani O, Feld R, Reed R (1996). "Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A.". Genes Dev. 10 (2): 233–43. PMID 8566756.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Das BK, Xia L, Palandjian L, et al. (2000). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.". Mol. Cell. Biol. 19 (10): 6796–802. PMID 10490618.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell 5 (5): 779–87. PMID 10882114.
Bryant HE, Wadd SE, Lamond AI, et al. (2001). "Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step.". J. Virol. 75 (9): 4376–85. doi:10.1128/JVI.75.9.4376-4385.2001. PMID 11287586.
Brand M, Moggs JG, Oulad-Abdelghani M, et al. (2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation.". EMBO J. 20 (12): 3187–96. doi:10.1093/emboj/20.12.3187. PMID 11406595.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMID 11500380.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.". RNA 8 (4): 426–39. PMID 11991638.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.". EMBO J. 21 (18): 4978–88. PMID 12234937.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Warner DR, Roberts EA, Greene RM, Pisano MM (2004). "Identification of novel Smad binding proteins.". Biochem. Biophys. Res. Commun. 312 (4): 1185–90. PMID 14651998.
Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.". RNA 10 (6): 929–41. PMID 15146077.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMID 15302935.
Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo.". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMID 15456888.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics.". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Ardon O, Zimmerman ES, Andersen JL, et al. (2006). "Induction of G2 arrest and binding to cyclophilin A are independent phenotypes of human immunodeficiency virus type 1 Vpr.". J. Virol. 80 (8): 3694–700. doi:10.1128/JVI.80.8.3694-3700.2006. PMID 16571786.