SF3B1

From Wikipedia, the free encyclopedia

Splicing factor 3b, subunit 1, 155kDa

PDB rendering based on 2f9d.

Available structures: 2f9d, 2f9j, 2fho

Identifiers

Symbol(s)

SF3B1; PRP10; PRPF10; SAP155; SF3b155

External IDs

OMIM: 605590 MGI: 1932339 HomoloGene: 6696

Gene ontology
Molecular Function:	• chromatin binding • protein binding • RNA splicing factor activity, transesterification mechanism
Cellular Component:	• nucleus • spliceosome
Biological Process:	• nuclear mRNA splicing, via spliceosome • RNA splicing • anterior/posterior pattern formation

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_001005526 (mRNA)
NP_001005526 (protein)

NM_031179 (mRNA)
NP_112456 (protein)

Location

Chr 2: 197.96 - 198.01 Mb

Chr 1: 54.93 - 54.97 Mb

Pubmed search

[1]

[2]

Splicing factor 3b, subunit 1, 155kDa, also known as SF3B1, is a human gene.^[1]

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.^[1]

[edit] References

^ ^a ^b Entrez Gene: SF3B1 splicing factor 3b, subunit 1, 155kDa.

[edit] Further reading

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353-8. PMID 9110174.
Wang C, Chua K, Seghezzi W, et al. (1998). "Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis.". Genes Dev. 12 (10): 1409-14. PMID 9585501.
Gozani O, Potashkin J, Reed R (1998). "A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.". Mol. Cell. Biol. 18 (8): 4752-60. PMID 9671485.
Das BK, Xia L, Palandjian L, et al. (2000). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.". Mol. Cell. Biol. 19 (10): 6796-802. PMID 10490618.
Pauling MH, McPheeters DS, Ares M (2000). "Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA.". Mol. Cell. Biol. 20 (6): 2176-85. PMID 10688664.
Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491-6. PMID 10737800.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell 5 (5): 779-87. PMID 10882114.
Brand M, Moggs JG, Oulad-Abdelghani M, et al. (2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation.". EMBO J. 20 (12): 3187-96. doi:10.1093/emboj/20.12.3187. PMID 11406595.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536-46. doi:10.1093/emboj/20.16.4536. PMID 11500380.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.". RNA 8 (4): 426-39. PMID 11991638.
Boudrez A, Beullens M, Waelkens E, et al. (2002). "Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1.". J. Biol. Chem. 277 (35): 31834-41. doi:10.1074/jbc.M204427200. PMID 12105215.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.". EMBO J. 21 (18): 4978-88. PMID 12234937.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Golas MM, Sander B, Will CL, et al. (2003). "Molecular architecture of the multiprotein splicing factor SF3b.". Science 300 (5621): 980-4. doi:10.1126/science.1084155. PMID 12738865.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039.
Banerjee H, Rahn A, Gawande B, et al. (2004). "The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.". RNA 10 (2): 240-53. PMID 14730023.
Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome.". RNA 10 (6): 929-41. PMID 15146077.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi:10.1073/pnas.0404720101. PMID 15302935.
Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo.". Mol. Cell. Biol. 24 (20): 9176-85. doi:10.1128/MCB.24.20.9176-9185.2004. PMID 15456888.