SF3A2

From Wikipedia, the free encyclopedia

Splicing factor 3a, subunit 2, 66kDa

Identifiers

SF3A2; PRP11; PRPF11; SAP62; SF3a66

External IDs

OMIM: 600796 MGI: 104912 HomoloGene: 5184

Gene ontology
Molecular Function:	• nucleic acid binding • zinc ion binding • metal ion binding
Cellular Component:	• intracellular • nucleus • spliceosome • small nuclear ribonucleoprotein complex
Biological Process:	• nuclear mRNA 3'-splice site recognition • nuclear mRNA splicing, via spliceosome • RNA splicing

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_007165 (mRNA)
NP_009096 (protein)

NM_013651 (mRNA)
NP_038679 (protein)

Location

Chr 19: 2.19 - 2.2 Mb

Chr 10: 80.2 - 80.21 Mb

Pubmed search

[1]

[2]

Splicing factor 3a, subunit 2, 66kDa, also known as SF3A2, is a human gene.^[1]

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.^[1]

[edit] References

^ ^a ^b Entrez Gene: SF3A2 splicing factor 3a, subunit 2, 66kDa.

[edit] Further reading

Chiara MD, Champion-Arnaud P, Buvoli M, et al. (1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114.". Proc. Natl. Acad. Sci. U.S.A. 91 (14): 6403-7. PMID 8022796.
Bennett M, Reed R (1993). "Correspondence between a mammalian spliceosome component and an essential yeast splicing factor.". Science 262 (5130): 105-8. PMID 8211113.
Dresser DW, Hacker A, Lovell-Badge R, Guerrier D (1996). "The genes for a spliceosome protein (SAP62) and the anti-Müllerian hormone (AMH) are contiguous.". Hum. Mol. Genet. 4 (9): 1613-8. PMID 8541848.
Rain JC, Tartakoff AM, Krämer A, Legrain P (1996). "Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution.". RNA 2 (6): 535-50. PMID 8718683.
Hong W, Bennett M, Xiao Y, et al. (1997). "Association of U2 snRNP with the spliceosomal complex E.". Nucleic Acids Res. 25 (2): 354-61. PMID 9016565.
Moraitou M, Patrinou-Georgoula M, Guialis A (1998). "Structural/functional properties of a mammalian multi-component structure containing all major spliceosomal small nuclear ribonucleoprotein particles.". Biochem. J. 332 ( Pt 1): 135-44. PMID 9576861.
Yan D, Perriman R, Igel H, et al. (1998). "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif.". Mol. Cell. Biol. 18 (9): 5000-9. PMID 9710584.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46-50. doi:10.1038/1700. PMID 9731529.
Krämer A, Grüter P, Gröning K, Kastner B (1999). "Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP.". J. Cell Biol. 145 (7): 1355-68. PMID 10385517.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E.". Mol. Cell 5 (5): 779-87. PMID 10882114.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.". EMBO J. 20 (16): 4536-46. doi:10.1093/emboj/20.16.4536. PMID 11500380.
Nesic D, Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.". Mol. Cell. Biol. 21 (19): 6406-17. PMID 11533230.
Dresser DW, Jamin SP, Atkins CJ, Guerrier D (2001). "An expressed GNRP-like gene shares a bi-directional promoter with SF3A2 (SAP62) immediately upstream of AMH.". Gene 277 (1-2): 163-73. PMID 11602354.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.". RNA 8 (4): 426-39. PMID 11991638.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.". EMBO J. 21 (18): 4978-88. PMID 12234937.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19.". Nature 428 (6982): 529-35. doi:10.1038/nature02399. PMID 15057824.
Takenaka K, Nakagawa H, Miyamoto S, Miki H (2005). "The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein.". Biochem. J. 382 (Pt 1): 223-30. doi:10.1042/BJ20040521. PMID 15142036.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway.". Genome Res. 14 (7): 1324-32. doi:10.1101/gr.2334104. PMID 15231748.
Nesic D, Tanackovic G, Krämer A (2005). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis.". J. Cell. Sci. 117 (Pt 19): 4423-33. doi:10.1242/jcs.01308. PMID 15316075.