RyhB RNA

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The figure from Tjaden et al. depicts the secondary structure for the RyhB RNA. The Sm-like protein Hfq binds to the AU-rich unstructured region of RyhB as indicated. Below the secondary structure, the primary sequence of RyhB is shown along with its putative binding interaction to the target mRNA sodB. The start codon for sodB is underlined. RyhB nucleotides that participate in the interaction are in bold.
The figure from Tjaden et al. depicts the secondary structure for the RyhB RNA. The Sm-like protein Hfq binds to the AU-rich unstructured region of RyhB as indicated. Below the secondary structure, the primary sequence of RyhB is shown along with its putative binding interaction to the target mRNA sodB. The start codon for sodB is underlined. RyhB nucleotides that participate in the interaction are in bold.[1]

RyhB RNA is a 90 nucleotide non-coding RNA that down-regulates a set of iron-storage and iron-using proteins when iron is limiting; it is itself negatively regulated by the ferric uptake repressor protein, Fur (Ferric uptake regulator). This ncRNA gene was recently identified in a screen and called SraI and was found to be expressed only in stationary phase.[2] RyhB RNA levels are inversely correlated with mRNA levels for the sdhCDAB operon, encoding succinate dehydrogenase, as well as five other genes previously shown to be positively regulated by Fur by an unknown mechanism. These include two other genes encoding enzymes in the tricarboxylic acid cycle, acnA and fumA, two ferritin genes, ftnA and bfr, and a gene for superoxide dismutase, sodB.[3] A number of other genes have been predicted computationally and verified as targets by microarray analysis: napF, sodA, cysE, yciS, acpS, nagZ and dadA.[1] RyhB is bound by the Hfq protein, that increases its interaction with its target messages.

[edit] References

  1. ^ a b Tjaden B, Goodwin SS, Opdyke JA, et al (2006). "Target prediction for small, noncoding RNAs in bacteria". Nucleic Acids Res. 34 (9): 2791–802. doi:10.1093/nar/gkl356. PMID 16717284. 
  2. ^ Argaman, L; Hershberg R, Vogel J, Bejerano G, Wagner EG, Margalit H, Altuvia S (2001). "Novel small RNA-encoding genes in the intergenic regions of Escherichia coli". Curr Biol 11: 941–950. doi:10.1016/S0960-9822(01)00270-6. PMID 11448770. 
  3. ^ Masse, E; Gottesman S (2002). "A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli". Proc Natl Acad Sci U S A 99: 4620–4625. doi:10.1073/pnas.032066599. PMID 11917098. 

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