Talk:RNA polymerase II
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[edit] Confused by the elongation part
I am confused by the elongation part of this article and I see no citations or references that would allow the text to be verifiable.
- Did you read the recent review by one of the experts on transcription regulation: Danny Reinberg? I think this would help the entire article a lot, especially the elongation part could be revised thoroughly.
- I don't understand the classification of elongation promoters?? Even if these are rather factors I don't follow it. Why did you make this division? What means Drug/sequence-dependent arrest affected factors? Why would factors that are affected by drugs be different from the ones that affect the catalytic activity of the RNA polymerase?
- What is the reference of There is, however, a change in methylation of the polymerase, midway through elongation the phosphorylation location changes slightly under the influence of Ctk1.? What is meant by the phosphorylation location? Do you mean a site on the CTD of the polymerase? --AAM 22:19, 19 February 2006 (UTC)
I removed the part on the methylation by Ctk1. Ctk1 is a kinase and these do not methylate. There might have been some confusion as phosphorylation of PolII by Ctk1 could affect the Set2 mediated histone methylation (see Genes Dev. 2003 Mar 1;17(5):654-63. Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.) If anybody comes with a reference proving that I was wrong in removing this text we can discuss. --User:AAM | Talk 16:08, 1 March 2006 (UTC)
[edit] Role of TATA box
The role of the TATA box is somewhat overestimated here. Relatively recent genome-scale studies in Homo sapiens and other species indicate that TATA boxes are NOT present in a majority of active promoters; e.g. see Bing Ren paper in Nature 2005. This is also, of course, supported by computational studies (i.e. searching for TATA boxes, not finding them...)
[edit] So... what is it?
This article tells me what RNA polymerase II does, but it doesn't say what it is - is it a kind of RNA? An organic chemical? An organelle? Same complaint for RNA polymerase I and RNA polymerase III. This is like writing an article on horse saying that a horse is ridden and used for farm work without ever saying that it is an animal! bd2412 T 18:28, 15 December 2006 (UTC)
I think the first sentence would be improved were it to read: "RNA polymerase II (also called RNAP II and Pol II) is an enzyme found in eukaryotic cells which transcribes DNA to synthesize precursors of mRNA and most hnRNA." This takes care of the essential basics which I feel are missing from this page.
It might also be useful to explain some of the abbreviations - for example hnRNA stands for 'histonal RNA'. 163.1.236.79 13:56, 10 February 2007 (UTC)
RNAPII is a protein/protein complex, not an organelle or a chemical. It is fairly clear what it is and RNAP is not an easy subject, so it is expected that it wouldn't be easy to understand. B —Preceding unsigned comment added by 146.115.19.108 (talk) 07:56, 20 December 2007 (UTC)
[edit] Picture?
I think this article would benefit from a picture/diagram of RNAP II. 203.160.122.99 21:26, 19 October 2007 (UTC)
- We might try "RNA polymerase (1i6h).png" from Wikimedia Commons. I can look into it further, but I think it's an image of yeast RNA polymerase II. Forluvoft 21:37, 19 October 2007 (UTC)
[edit] New category to replace DNA Replication category?
Shouldn't this article and others related to transcription be re-categorized to a new category, Category:DNA transcription? Category:DNA replication doesn't make sense to me. —Preceding unsigned comment added by Skoch3 (talk • contribs) 05:59, 11 January 2008 (UTC)
