Ribosylpyrimidine nucleosidase

From Wikipedia, the free encyclopedia

In enzymology, a ribosylpyrimidine nucleosidase (EC 3.2.2.8) is an enzyme that catalyzes the chemical reaction

a pyrimidine nucleoside + H₂O D-ribose + a pyrimidine base

Thus, the two substrates of this enzyme are pyrimidine nucleoside and H₂O, whereas its two products are D-ribose and pyrimidine base.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is pyrimidine-nucleoside ribohydrolase. Other names in common use include N-ribosylpyrimidine nucleosidase, pyrimidine nucleosidase, N-ribosylpyrimidine ribohydrolase, pyrimidine nucleoside hydrolase, RihB, YeiK, and nucleoside ribohydrolase. This enzyme participates in purine metabolism and pyrimidine metabolism.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1Q8F and 1YOE.

[edit] References

• IUBMB entry for 3.2.2.8
• BRENDA references for 3.2.2.8 (Recommended.)
• PubMed references for 3.2.2.8
• PubMed Central references for 3.2.2.8
• Google Scholar references for 3.2.2.8
• Terada M, Tatibana M, Hayaishi O (1967). "Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens". J. Biol. Chem. 242: 5578–85. PMID 12325375. 
• Petersen C, Moller LB (2001). "The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation". J. Biol. Chem. 276: 884–94. doi:10.1074/jbc.M008300200. PMID 11027694. 
• Giabbai B, Degano M (2004). "Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK". Acta. Crystallogr. D. Biol. Crystallogr. 60: 524–7. doi:10.1107/S0907444903028488. PMID 14993681. 
• Giabbai B, Degano M (2004). "Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy". Structure. 12: 739–49. doi:10.1016/j.str.2004.03.018. PMID 15130467. 

[edit] External links

The CAS registry number for this enzyme class is 37288-60-1.

• IUBMB entry for 3.2.2.8

• KEGG entry for 3.2.2.8

• BRENDA entry for 3.2.2.8

• NiceZyme view of 3.2.2.8

• EC2PDB: PDB structures for 3.2.2.8

• PRIAM entry for 3.2.2.8

• PUMA2 entry for 3.2.2.8

• IntEnz: Integrated Enzyme entry for 3.2.2.8

• MetaCyc entry for 3.2.2.8

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0050263: ribosylpyrimidine nucleosidase

• AMIGO entry for GO code 0050263: ribosylpyrimidine nucleosidase