RFC5

From Wikipedia, the free encyclopedia

Replication factor C (activator 1) 5, 36.5kDa

Identifiers

RFC5; MGC1155; RFC36

External IDs

Gene ontology
Molecular Function:	• nucleotide binding • ATP binding • nucleoside-triphosphatase activity • enzyme binding
Cellular Component:	• nucleus • DNA replication factor C complex
Biological Process:	• DNA replication • DNA repair

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

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Refseq

XM_001132724 (mRNA)
XP_001132724 (protein)

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n/a (protein)

Location

Chr 12: 116.94 - 116.95 Mb

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Pubmed search

[1]

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Replication factor C (activator 1) 5, 36.5kDa, also known as RFC5, is a human gene.^[1]

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.^[1]

[edit] References

^ ^a ^b Entrez Gene: RFC5 replication factor C (activator 1) 5, 36.5kDa.

[edit] Further reading

Okumura K, Nogami M, Taguchi H, et al. (1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23.". Genomics 25 (1): 274–8. PMID 7774928.
O'Donnell M, Onrust R, Dean FB, et al. (1993). "Homology in accessory proteins of replicative polymerases--E. coli to humans.". Nucleic Acids Res. 21 (1): 1–3. PMID 8441605.
Uhlmann F, Cai J, Flores-Rozas H, et al. (1996). "In vitro reconstitution of human replication factor C from its five subunits.". Proc. Natl. Acad. Sci. U.S.A. 93 (13): 6521–6. PMID 8692848.
Mossi R, Jónsson ZO, Allen BL, et al. (1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen.". J. Biol. Chem. 272 (3): 1769–76. PMID 8999859.
Cujec TP, Cho H, Maldonado E, et al. (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.". Mol. Cell. Biol. 17 (4): 1817–23. PMID 9121429.
Cai J, Gibbs E, Uhlmann F, et al. (1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme.". J. Biol. Chem. 272 (30): 18974–81. PMID 9228079.
Loor G, Zhang SJ, Zhang P, et al. (1998). "Identification of DNA replication and cell cycle proteins that interact with PCNA.". Nucleic Acids Res. 25 (24): 5041–6. PMID 9396813.
Ellison V, Stillman B (1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity.". J. Biol. Chem. 273 (10): 5979–87. PMID 9488738.
Cai J, Yao N, Gibbs E, et al. (1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits.". Proc. Natl. Acad. Sci. U.S.A. 95 (20): 11607–12. PMID 9751713.
Zhang G, Gibbs E, Kelman Z, et al. (1999). "Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen.". Proc. Natl. Acad. Sci. U.S.A. 96 (5): 1869–74. PMID 10051561.
Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (2001). "Purification and characterization of human DNA damage checkpoint Rad complexes.". Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11236–41. doi:10.1073/pnas.201373498. PMID 11572977.
Griffith JD, Lindsey-Boltz LA, Sancar A (2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy.". J. Biol. Chem. 277 (18): 15233–6. doi:10.1074/jbc.C200129200. PMID 11907025.
Ohta S, Shiomi Y, Sugimoto K, et al. (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein.". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
Maruyama T, Farina A, Dey A, et al. (2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase.". Mol. Cell. Biol. 22 (18): 6509–20. PMID 12192049.
Venclovas C, Colvin ME, Thelen MP (2003). "Molecular modeling-based analysis of interactions in the RFC-dependent clamp-loading process.". Protein Sci. 11 (10): 2403–16. PMID 12237462.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment.". J. Biol. Chem. 278 (32): 30051–6. doi:10.1074/jbc.M211591200. PMID 12766176.
Bermudez VP, Maniwa Y, Tappin I, et al. (2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA.". Proc. Natl. Acad. Sci. U.S.A. 100 (18): 10237–42. doi:10.1073/pnas.1434308100. PMID 12930902.
Zou L, Liu D, Elledge SJ (2004). "Replication protein A-mediated recruitment and activation of Rad17 complexes.". Proc. Natl. Acad. Sci. U.S.A. 100 (24): 13827–32. doi:10.1073/pnas.2336100100. PMID 14605214.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.