Talk:Real-time polymerase chain reaction

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Contents 1 Confusion on Methods 2 Images 3 Merge/Link with qPCR? 4 Merge with Real-time PCR/Temp 5 Reverse transcription pcr? 6 Cool Real Time PCR site

[edit] Confusion on Methods

Does real-time PCR use FRET or some other kind of energy transfer? Heid's paper "Real Time Quantitative PCR" only refers to it as "fluorescent energy transfer." However, the wiki page on FRET states that it is NOT in fact fluorescence as it is not a "radiative" energy emission. Any help clearing this up? —Preceding unsigned comment added by 146.189.246.13 (talk) 15:20, 16 May 2008 (UTC)

[edit] Images

While the images look very pretty, they are also pretty inaccurate. The DNAs depicted are left-handed and have no major or minor grooves. I don't know if aesthetics or complete accuracy is more important here. I've also moved one image, since it had been badly integrated into the text. --163.1.176.254 14:25, 25 May 2007 (UTC)

[edit] Merge/Link with qPCR?

A lot of duplication exists between qPCR and this page. Admittedly they are not exactly the same, but some links should be made between the two. Any comments? Dr Aaron 23:05, 1 August 2006 (UTC)

I am not an expert, but I think that the "Fluorescent reporter probe method" of qPCR is the same as RT-PCR using a probe with reporter and quencher. Oderbolz 08:12, 21 February 2007 (UTC)

[edit] Merge with Real-time PCR/Temp

Needs merging with Real-time PCR/Temp —Preceding unsigned comment added by Zephyris (talk • contribs)

Merging done. Elle _{vécut heureuse} ^{à jamais} (Be eudaimonic!) 23:47, 16 February 2006 (UTC)

[edit] Reverse transcription pcr?

Much of the information on this page seems more related to reverse transcription pcr - I understand real time pcr has applications in fields such as ancient DNA and forensics? Aaadddaaammm 22:27, 31 January 2007 (UTC)

There seems to be a confusion of acronyms: RT-PCR is either "Real Time" PCR (Quantitative) or "Reverse Transcriptase" PCR (Qualitative PCR for RNA), I think we need to distinguish these concepts. Oderbolz 08:15, 21 February 2007 (UTC)

[edit] Cool Real Time PCR site

Helped me recently with real time pcr problems: Real Time PCR Dnaguys cheers!