RBX1

From Wikipedia, the free encyclopedia

Ring-box 1

PDB rendering based on 1ldj.

Available structures: 1ldj, 1ldk, 1u6g, 2hye

Identifiers

Symbol(s)

RBX1; ROC1; BA554C12.1; MGC13357; MGC1481; RNF75

External IDs

OMIM: 603814 MGI: 1891829 HomoloGene: 6872

Gene ontology
Molecular Function:	• protein binding • zinc ion binding • metal ion binding
Cellular Component:	• nucleus
Biological Process:	• DNA repair • ubiquitin cycle • protein catabolic process

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_014248 (mRNA)
NP_055063 (protein)

NM_019712 (mRNA)
NP_062686 (protein)

Location

Chr 22: 39.68 - 39.7 Mb

Chr 15: 81.29 - 81.3 Mb

Pubmed search

[1]

[2]

Ring-box 1, also known as RBX1, is a human gene.

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.^[1]

[edit] References

^ Entrez Gene: RBX1 ring-box 1.

[edit] Further reading

Kamura T, Koepp DM, Conrad MN, et al. (1999). "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.". Science 284 (5414): 657-61. PMID 10213691.
Skowyra D, Koepp DM, Kamura T, et al. (1999). "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.". Science 284 (5414): 662-5. PMID 10213692.
Tan P, Fuchs SY, Chen A, et al. (1999). "Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.". Mol. Cell 3 (4): 527-33. PMID 10230406.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y (1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.". Mol. Cell 3 (4): 535-41. PMID 10230407.
Winston JT, Koepp DM, Zhu C, et al. (1999). "A family of mammalian F-box proteins.". Curr. Biol. 9 (20): 1180-2. doi:10.1016/S0960-9822(00)80021-4. PMID 10531037.
Kamura T, Conrad MN, Yan Q, et al. (2000). "The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2.". Genes Dev. 13 (22): 2928-33. PMID 10579999.
Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22.". Nature 402 (6761): 489-95. doi:10.1038/990031. PMID 10591208.
Périn J-P , Seddiqi N, Charbonnier F, et al. (2000). "Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1.". Cell. Mol. Biol. (Noisy-le-grand) 45 (8): 1131-7. PMID 10643962.
Read MA, Brownell JE, Gladysheva TB, et al. (2000). "Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha.". Mol. Cell. Biol. 20 (7): 2326-33. PMID 10713156.
Furukawa M, Zhang Y, McCarville J, et al. (2000). "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1.". Mol. Cell. Biol. 20 (21): 8185-97. PMID 11027288.
Maeda I, Ohta T, Koizumi H, Fukuda M (2001). "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3.". FEBS Lett. 494 (3): 181-5. PMID 11311237.
Lyapina S, Cope G, Shevchenko A, et al. (2001). "Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.". Science 292 (5520): 1382-5. doi:10.1126/science.1059780. PMID 11337588.
Fukuchi M, Imamura T, Chiba T, et al. (2001). "Ligand-dependent degradation of Smad3 by a ubiquitin ligase complex of ROC1 and associated proteins.". Mol. Biol. Cell 12 (5): 1431-43. PMID 11359933.
Kamura T, Burian D, Yan Q, et al. (2001). "Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.". J. Biol. Chem. 276 (32): 29748-53. doi:10.1074/jbc.M103093200. PMID 11384984.
Wu K, Chen A, Tan P, Pan ZQ (2002). "The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.". J. Biol. Chem. 277 (1): 516-27. doi:10.1074/jbc.M108008200. PMID 11675391.
Gomes MD, Lecker SH, Jagoe RT, et al. (2002). "Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy.". Proc. Natl. Acad. Sci. U.S.A. 98 (25): 14440-5. doi:10.1073/pnas.251541198. PMID 11717410.
Kim J, Kim JH, Lee SH, et al. (2002). "The novel human DNA helicase hFBH1 is an F-box protein.". J. Biol. Chem. 277 (27): 24530-7. doi:10.1074/jbc.M201612200. PMID 11956208.
Zheng N, Schulman BA, Song L, et al. (2002). "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex.". Nature 416 (6882): 703-9. doi:10.1038/416703a. PMID 11961546.
Yoshida Y, Chiba T, Tokunaga F, et al. (2002). "E3 ubiquitin ligase that recognizes sugar chains.". Nature 418 (6896): 438-42. doi:10.1038/nature00890. PMID 12140560.
Brower CS, Sato S, Tomomori-Sato C, et al. (2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.". Proc. Natl. Acad. Sci. U.S.A. 99 (16): 10353-8. doi:10.1073/pnas.162424199. PMID 12149480.