Talk:Rapid Amplification of cDNA Ends
From Wikipedia, the free encyclopedia
 |
This article is within the scope of the Molecular and Cellular Biology WikiProject. To participate, visit the WikiProject for more information. The WikiProject's current monthly collaboration is focused on improving Restriction enzyme. |
||
| Stub | This article has been rated as stub-Class on the assessment scale. | ||
|
Article Grading: The article has been rated for quality and/or importance but has no comments yet. If appropriate, please review the article and then leave comments here to identify the strengths and weaknesses of the article and what work it will need. |
|||
[edit] Edit on 3 April 2008
I wrote an introduction and removed the context tag. Not sure if I'm supposed to remove it myself or wait for another editor to remove it. Maybe somebody can let me know.
The article needs a figure to illustrate the strands and the amplifications. I had to draw one myself when I read the article in order to understand it. Drummstikk (talk) 01:44, 4 April 2008 (UTC)
[edit] 5' RACE edit of 23 Oct 2007
I fixed the description of 5' RACE. I think confusion stemmed from the fact that the cDNA strand synthesized (akin to first strand synthesis in RT-PCR) is antisense.
TdT adds a poly-T tail to the 3' end of the newly synthesized cDNA. One, because TdT acts on the 3' ends of DNA. Two, because adding a known priming site to the 5' end of the newly synthesized antisense strand would attach it to the end of the known sequence. (The 5' end of the antisense cDNA corresponds to the known, or at least not yet desired, 3' end of the mRNA.)
Kwedin 01:35, 24 October 2007 (UTC)
[edit] Article title
There's already a "Race (biology)" article discussing races of animals. This article is currently titled "RACE (biology)". This doesn't seem very distinctive to me. Can I suggest either RACE (molecular biology) or RACE (Rapid Amplification of cDNA Ends)?
Kwedin 01:40, 24 October 2007 (UTC)
- How about Rapid Amplification of cDNA Ends, we can avoid parantheses alltogether--ZayZayEM 02:38, 24 October 2007 (UTC)
