RAD18

From Wikipedia, the free encyclopedia

RAD18 homolog (S. cerevisiae)

Identifiers

External IDs

OMIM: 605256 MGI: 1890476 HomoloGene: 48572

Gene ontology
Molecular Function:	• damaged DNA binding • protein binding • zinc ion binding • metal ion binding
Cellular Component:	• chromatin • nucleus
Biological Process:	• DNA repair

Orthologs

Human

Mouse

Refseq

NM_020165 (mRNA)
NP_064550 (protein)

NM_021385 (mRNA)
NP_067360 (protein)

Location

Chr 3: 8.89 - 8.98 Mb

Chr 6: 112.59 - 112.66 Mb

Pubmed search

[1]

[2]

RAD18 homolog (S. cerevisiae), also known as RAD18, is a human gene.^[1]

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is a ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.^[1]

[edit] References

^ ^a ^b Entrez Gene: RAD18 RAD18 homolog (S. cerevisiae).

[edit] Further reading

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171-4. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149-56. PMID 9373149.
Tateishi S, Sakuraba Y, Masuyama S, et al. (2000). "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.". Proc. Natl. Acad. Sci. U.S.A. 97 (14): 7927-32. PMID 10884424.
Xin H, Lin W, Sumanasekera W, et al. (2000). "The human RAD18 gene product interacts with HHR6A and HHR6B.". Nucleic Acids Res. 28 (14): 2847-54. PMID 10908344.
Mulder LC, Chakrabarti LA, Muesing MA (2002). "Interaction of HIV-1 integrase with DNA repair protein hRad18.". J. Biol. Chem. 277 (30): 27489-93. doi:10.1074/jbc. M203061200. PMID 12016221.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Nikiforov AA, Sasina LK, Svetlova MP, et al. (2004). "Early immobilization of nuclease FEN1 and accumulation of hRAD18 protein at stalled DNA replication forks in mammalian cells.". Dokl. Biochem. Biophys. 389: 122-5. PMID 12856420.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi:10.1073/pnas.0404720101. PMID 15302935.
Watanabe K, Tateishi S, Kawasuji M, et al. (2005). "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.". EMBO J. 23 (19): 3886-96. doi:10.1038/sj.emboj.7600383. PMID 15359278.
Nikiforov A, Svetlova M, Solovjeva L, et al. (2004). "DNA damage-induced accumulation of Rad18 protein at stalled replication forks in mammalian cells involves upstream protein phosphorylation.". Biochem. Biophys. Res. Commun. 323 (3): 831-7. doi:10.1016/j.bbrc.2004.08.165. PMID 15381075.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.
Miyase S, Tateishi S, Watanabe K, et al. (2005). "Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination.". J. Biol. Chem. 280 (1): 515-24. doi:10.1074/jbc. M409219200. PMID 15509568.
Masuyama S, Tateishi S, Yomogida K, et al. (2005). "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions.". Genes Cells 10 (8): 753-62. doi:10.1111/j.1365-2443.2005.00874.x. PMID 16098139.
Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle.". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391-6. doi:10.1073/pnas.0507066103. PMID 16565220.
Bi X, Barkley LR, Slater DM, et al. (2006). "Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest.". Mol. Cell. Biol. 26 (9): 3527-40. doi:10.1128/MCB.26.9.3527-3540.2006. PMID 16611994.
Lloyd AG, Tateishi S, Bieniasz PD, et al. (2006). "Effect of DNA repair protein Rad18 on viral infection.". PLoS Pathog. 2 (5): e40. doi:10.1371/journal.ppat.0020040. PMID 16710452.
Yuasa MS, Masutani C, Hirano A, et al. (2006). "A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest.". Genes Cells 11 (7): 731-44. doi:10.1111/j.1365-2443.2006.00974.x. PMID 16824193.
Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization.". Nat. Biotechnol. 24 (10): 1285-92. doi:10.1038/nbt1240. PMID 16964243.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.". Cell 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.