QUAD RNA

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QUAD RNA

Type:	Gene; sRNA;
2° structure:	Predicted; PFOLD
Seed alignment:	Gottesman S, Storz G
Avg length:	146.9 nucleotides
Avg identity:	77%

QUAD RNAs refers to a group of related non-coding RNA (ncRNA) identified in the bacteria Escherichia coli but their function is unknown.

These small RNA were identified computationally by searching the genome of E. coli for intergenic regions of high sequence identity (sequence conservation) with the genomes of closely related bacteria (several salmonella species and Klebsiella pneumoniae)^[1]. This data was combined with microarray expression analysis and potential novel ncRNAs identified. The expression of novel ncRNA of interest was confirmed by northern blotting.

In this large scale screen these ncRNAs were simply referred to as candidates 43,55 and 61^[1]. These 3 ncRNA appear to be highly homologous and are derived from a repeat region of the genome. These RNA were identified in another study ^[2] Each of the ncRNA contains a short stretch homologous to boxC, a repeat element of unknown function present in 50 copies or more within the genome of E. coli ^[3]

[edit] References

^ ^a ^b Wassarman KM, Repoila F, Rosenow C, Storz G, Gottesman S (2001). "Identification of novel small RNAs using comparative genomics and microarrays". Genes Dev. 15 (13): 1637–51. doi:10.1101/gad.901001. PMID 11445539.
^ Rudd KE (1999). "Novel intergenic repeats of Escherichia coli K-12". Res. Microbiol. 150 (9-10): 653–64. PMID 10673004.
^ Bachellier, S., Gilson, E., Hofnung, M., and Hill, C.W. 1996. Repeated sequences. In Escherichia coli and Salmonella: Cellular and molecular biology (ed. F.C. Neidhardt, et al.), pp. 2012-2040. American Society for Microbiology, Washington, D.C.