Pyruvate, water dikinase

From Wikipedia, the free encyclopedia

In enzymology, a pyruvate, water dikinase (EC 2.7.9.2) is an enzyme that catalyzes the chemical reaction

ATP + pyruvate + H₂O AMP + phosphoenolpyruvate + phosphate

The 3 substrates of this enzyme are ATP, pyruvate, and H₂O, whereas its 3 products are AMP, phosphoenolpyruvate, and phosphate.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with paired acceptors. The systematic name of this enzyme class is ATP:pyruvate, water phosphotransferase. Other names in common use include phosphoenolpyruvate synthase, pyruvate-water dikinase (phosphorylating), PEP synthetase, phosphoenolpyruvate synthase, phoephoenolpyruvate synthetase, phosphoenolpyruvic synthase, and phosphopyruvate synthetase. This enzyme participates in pyruvate metabolism and reductive carboxylate cycle (co2 fixation). It employs one cofactor, manganese.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2OLS.

[edit] References

• IUBMB entry for 2.7.9.2
• BRENDA references for 2.7.9.2 (Recommended.)
• PubMed references for 2.7.9.2
• PubMed Central references for 2.7.9.2
• Google Scholar references for 2.7.9.2
• Berman KM, Cohn M (1970). "Phosphoenolpyruvate synthetase of Escherichia coli. Purification, some properties, and the role of divalent metal ions". J. Biol. Chem. 245: 5309–18. PMID 4319237. 
• Berman KM, Cohn M (1970). "Phosphoenolpyruvate synthetase. Partial reactions studied with adenosine triphosphate analogues and the inorganic phosphate-H2 18O exchange reaction". J. Biol. Chem. 245: 5319–25. PMID 4319238. 
• Cooper RA, Kornberg HL (1965). "Net formation of phosphoenolpyruvate from pyruvate by Escherichia coli". Biochim. Biophys. Acta. 104: 618–20. PMID 5322808. 
• Cooper RA and Kornberg HL (1969). "Phosphoenolpyruvate synthetase". Methods Enzymol. 13: 309–314. 

[edit] External links

The CAS registry number for this enzyme class is 9013-09-6.

• IUBMB entry for 2.7.9.2

• KEGG entry for 2.7.9.2

• BRENDA entry for 2.7.9.2

• NiceZyme view of 2.7.9.2

• EC2PDB: PDB structures for 2.7.9.2

• PRIAM entry for 2.7.9.2

• PUMA2 entry for 2.7.9.2

• IntEnz: Integrated Enzyme entry for 2.7.9.2

• MetaCyc entry for 2.7.9.2

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0008986: pyruvate, water dikinase

• AMIGO entry for GO code 0008986: pyruvate, water dikinase