Pyrimidine-nucleoside phosphorylase

From Wikipedia, the free encyclopedia

In enzymology, a pyrimidine-nucleoside phosphorylase (EC 2.4.2.2) is an enzyme that catalyzes the chemical reaction

a pyrimidine nucleoside + phosphate $\rightleftharpoons$ a pyrimidine base + alpha-D-ribose 1-phosphate

Thus, the two substrates of this enzyme are pyrimidine nucleoside and phosphate, whereas its two products are pyrimidine base and alpha-D-ribose 1-phosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is pyrimidine-nucleoside:phosphate alpha-D-ribosyltransferase. This enzyme is also called Py-NPase. This enzyme participates in pyrimidine metabolism.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1BRW and 2DSJ.

[edit] References

IUBMB entry for 2.4.2.2
BRENDA references for 2.4.2.2 (Recommended.)
PubMed references for 2.4.2.2
PubMed Central references for 2.4.2.2
Google Scholar references for 2.4.2.2
Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York, 1961, p. 237-255.
Saunders PP, Wilson BA, Saunders GF (1969). "Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus". J. Biol. Chem. 244: 3691–7. PMID 4978445.
Hamamoto T, Noguchi T, Midorikawa Y (1996). "Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2". Biosci. Biotechnol. Biochem. 60: 1179–80. PMID 8782414.