Purine nucleosidase

From Wikipedia, the free encyclopedia

In enzymology, a purine nucleosidase (EC 3.2.2.1) is an enzyme that catalyzes the chemical reaction

a purine nucleoside + H₂O D-ribose + a purine base

Thus, the two substrates of this enzyme are purine nucleoside and H₂O, whereas its two products are D-ribose and purine base.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is purine-nucleoside ribohydrolase. Other names in common use include nucleosidase, purine beta-ribosidase, purine nucleoside hydrolase, purine ribonucleosidase, ribonucleoside hydrolase, nucleoside hydrolase, N-ribosyl purine ribohydrolase, nucleosidase g, N-D-ribosylpurine ribohydrolase, inosine-adenosine-guanosine preferring nucleoside hydrolase, purine-specific nucleoside N-ribohydrolase, IAG-nucleoside hydrolase, and IAG-NH. This enzyme participates in purine metabolism and nicotinate and nicotinamide metabolism.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 11 structures have been solved for this class of enzymes, with PDB accession codes 1EZR, 1HOZ, 1HP0, 1KIC, 1KIE, 1MAS, 1R4F, 2C40, 2FF1, 2FF2, and 2MAS.

[edit] References

• IUBMB entry for 3.2.2.1
• BRENDA references for 3.2.2.1 (Recommended.)
• PubMed references for 3.2.2.1
• PubMed Central references for 3.2.2.1
• Google Scholar references for 3.2.2.1
• HEPPEL LA, HILMOE RJ (1952). "[Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.]". J. Biol. Chem. 198: 683–94. PMID 12999785. 
• Kalckar HM (Napoli). "Biosynthetic aspects of nucleosides and nucleic acids". Pubbl. Staz. Zool.: 87–103. 
• Takagi Y and Horecker BL (1956). "Purification and properties of a bacterial riboside hydrolyase". J. Biol. Chem. 225: 77–86. 
• Tarr HLA (1955). "Fish muscle riboside hydrolases". Biochem. J. 59: 386–391. 
• Parkin DW (1996). "Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism". J. Biol. Chem. 271: 21713–9. PMID 8702965. 
• S (2001). "Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi". Appl. Environ. Microbiol. 67: 1783–7. doi:10.1128/AEM.67.4.1783-1787.2001. PMID 11282633. 
• Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J (2002). "Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax". J. Biol. Chem. 277: 15938–46. doi:10.1074/jbc.M111735200. PMID 11854281. 
• Mazumder-Shivakumar D, Bruice TC (2005). "Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues". Biochemistry. 44: 7805–17. doi:10.1021/bi047394h. PMID 15909995. 

[edit] External links

The CAS registry number for this enzyme class is 9025-44-9.

• IUBMB entry for 3.2.2.1

• KEGG entry for 3.2.2.1

• BRENDA entry for 3.2.2.1

• NiceZyme view of 3.2.2.1

• EC2PDB: PDB structures for 3.2.2.1

• PRIAM entry for 3.2.2.1

• PUMA2 entry for 3.2.2.1

• IntEnz: Integrated Enzyme entry for 3.2.2.1

• MetaCyc entry for 3.2.2.1

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0008477: purine nucleosidase

• AMIGO entry for GO code 0008477: purine nucleosidase