Protein sequencer

From Wikipedia, the free encyclopedia

A protein sequencer is a machine that is used to determine the sequence of amino acids in a protein.

They work by tagging and removing one amino acid at a time, which is analysed and identified. This is done repetitively for the whole polypeptide, until the whole sequence is established.

This method has generally been replaced by nucleic acid technology, and it is often easier to identify the sequence of a protein by looking at the DNA that codes for it.

v • d • e Proteins: key methods of study Experimental Protein purification - Green fluorescent protein - Western blot - Protein immunostaining - Protein sequencing - Gel electrophoresis/Protein electrophoresis - Protein immunoprecipitation - Peptide mass fingerprinting Bioinformatics Protein structure prediction - Protein-protein docking - Protein structural alignment - Protein ontology - Protein-protein interaction prediction Assay Enzyme assay - Protein assay - Secretion assay