PRKAG3

From Wikipedia, the free encyclopedia

Protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Identifiers

External IDs

OMIM: 604976 MGI: 1891343 HomoloGene: 23006

Gene ontology
Molecular Function:	• AMP-activated protein kinase activity
Biological Process:	• glycogen biosynthetic process • generation of precursor metabolites and energy • fatty acid biosynthetic process • protein kinase cascade • glucose transport

Orthologs

Human

Mouse

Refseq

NM_017431 (mRNA)
NP_059127 (protein)

XM_992465 (mRNA)
XP_997559 (protein)

Location

Chr 2: 219.4 - 219.4 Mb

Chr 1: 74.67 - 74.68 Mb

Pubmed search

[1]

[2]

Protein kinase, AMP-activated, gamma 3 non-catalytic subunit, also known as PRKAG3, is a human gene.^[1]

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.^[1]

[edit] References

^ ^a ^b Entrez Gene: PRKAG3 protein kinase, AMP-activated, gamma 3 non-catalytic subunit.

[edit] Further reading

Woods A, Cheung PC, Smith FC, et al. (1996). "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro.". J. Biol. Chem. 271 (17): 10282–90. PMID 8626596.
Cheung PC, Salt IP, Davies SP, et al. (2000). "Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding.". Biochem. J. 346 Pt 3: 659–69. PMID 10698692.
Milan D, Jeon JT, Looft C, et al. (2000). "A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle.". Science 288 (5469): 1248–51. PMID 10818001.
Nielsen JN, Mustard KJ, Graham DA, et al. (2003). "5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle.". J. Appl. Physiol. 94 (2): 631–41. doi:10.1152/japplphysiol.00642.2002. PMID 12391032.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Jeon JT, Park EW, Jeon HJ, et al. (2004). "A large-insert porcine library with sevenfold genome coverage: a tool for positional cloning of candidate genes for major quantitative traits.". Mol. Cells 16 (1): 113–6. PMID 14503854.
Mahlapuu M, Johansson C, Lindgren K, et al. (2004). "Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle.". Am. J. Physiol. Endocrinol. Metab. 286 (2): E194–200. doi:10.1152/ajpendo.00147.2003. PMID 14559719.
Amarger V, Erlandsson R, Pielberg G, et al. (2004). "Comparative sequence analysis of the PRKAG3 region between human and pig: evolution of repetitive sequences and potential new exons.". Cytogenet. Genome Res. 102 (1-4): 163–72. doi:10.1159/000075743. PMID 14970697.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Barnes BR, Long YC, Steiler TL, et al. (2006). "Changes in exercise-induced gene expression in 5'-AMP-activated protein kinase gamma3-null and gamma3 R225Q transgenic mice.". Diabetes 54 (12): 3484–9. PMID 16306365.
Lindgren K, Ormestad M, Persson M, et al. (2007). "Regulation of the muscle-specific AMP-activated protein kinase alpha2beta2gamma3 complexes by AMP and implications of the mutations in the gamma3-subunit for the AMP dependence of the enzyme.". FEBS J. 274 (11): 2887–96. doi:10.1111/j.1742-4658.2007.05821.x. PMID 17518971.