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The Principality of Anhalt-Mühlingen was a part of the Holy Roman Empire. It was created in 1667 following the death of Prince John VI and the partition of Anhalt-Zerbst with Anhalt-Mühlingen being created along with Anhalt-Dornburg for the younger sons of Prince John VI. There was only ever one Prince of Anhalt-Mühlingen as Prince Anthony Günther only had a daughter and so with his death in 1714 the Anhalt-Mühlingen line of the House of Ascania became extinct.
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