Talk:Polyhistidine-tag
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Original text:
- A his-tag is a technique used in biochemistry to purify proteins. The protein to purify is expressed as a fusion protein, carrrying a series of histidine residues at either the amino or the carboxyl end. The name-giving histidine residues will stick to a nickel surface, and can be eluted from there via imidazol. His-tagged proteins can also be identified on a Western blot via α-his-antibodies.
dekay 11:13, 18 Aug 2004 (UTC)
[edit] Generic name
I've revised this to say "polyhistidine-tag", which is apparently the correct generic name, rather than "his-tag", which EMD Biosciences are claiming is a trademark. This isn't a topic I know much about, so please let me know on my talk page if there is any problem with this change. Angela. 21:42, 15 October 2005 (UTC)
[edit] Generic name?
- User Angela moved the his tag article to polyhistidine tag, because of a trademark issue. I have written several parts of the original article and I don't agree that a his-tag is the same as the poly histidine tag. The purification and detection methods that described in this article work for the his tag, but I am not whether everything can transferred to the poly histidine tag. The copy paste edit from Angela did not improve this article, but made it rather confusing by mixing things up.
- The term his-tag is the most frequently used term in scientific literature and the term poly histidine tag is used less frequent.
- The trademark name for the His tag is 6xHis tag (Qiagen, which holds an exclusive licence for parts of the technology)
- Poly histidine tag is a copy cat approach of the his tag and is a possible infringment of the original intelectual property owned by Roche.
- his tag or better the 6xhis tag is not well discribed by the term poly histidine tag becausethe latin prefix poly leterally means many which is not the case for six histitdines the appropriate word might be oligo his tag (but that is not currently used in scientific literature)
JOK 11:38, 25 October 2005 (UTC)
- Please fix whatever factual issues there are here and explain whatever minor differences there may be between the generic and non-generic versions. Here is the email that the foundation just got from EMD:
The "Polyhistidine-tag" entry provided by your link below is fine, however I note that "His Tag" (without a hyphen) is still listed as an entry in your encyclopedia. Furthermore, after discussing this issue internally, we are now agreeable to have Wikipedia refer to our trademark (His-Tag®), as long as Wikipedia makes it clear that this trademark is owned by EMD Biosciences. Therefore I would like to ask that you remove the His Tag entry from your encyclopedia and transfer any pertinent information to the Polyhistidine-tag entry, making sure that any remaining reference to His-Tag clearly indicate that this registered trademark belongs to EMD Biosciences. I look forward to your reply and resolving this issue to mutual satisfaction. Regards, Marie Marie Azzaria, Ph.D. Manager, Intellectual Property
- The way things are now is fine. I don't want to deal with this issue again. Recreating the separate article at the registered trademark name may put the foundation in needless legal jeopardy. So please don't do that. --mav 03:39, 29 October 2005 (UTC)
