Polynucleotide 3'-phosphatase

From Wikipedia, the free encyclopedia

In enzymology, a polynucleotide 3'-phosphatase (EC 3.1.3.32) is an enzyme that catalyzes the chemical reaction

a 3'-phosphopolynucleotide + H₂O a polynucleotide + phosphate

Thus, the two substrates of this enzyme are 3'-phosphopolynucleotide and H₂O, whereas its two products are polynucleotide and phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is polynucleotide 3'-phosphohydrolase. Other names in common use include 2'(3')-polynucleotidase, DNA 3'-phosphatase, deoxyribonucleate 3'-phosphatase, and 5'-polynucleotidekinase 3'-phosphatase.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1UJX and 2BRF.

[edit] References

• IUBMB entry for 3.1.3.32
• BRENDA references for 3.1.3.32 (Recommended.)
• PubMed references for 3.1.3.32
• PubMed Central references for 3.1.3.32
• Google Scholar references for 3.1.3.32
• Becker A, Hurwitz J (1967). "The enzymatic cleavage of phosphate termini from polynucleotides". J. Biol. Chem. 242: 936–50. PMID 4289819. 

[edit] External links

The CAS registry number for this enzyme class is 37288-16-7.

• IUBMB entry for 3.1.3.32

• KEGG entry for 3.1.3.32

• BRENDA entry for 3.1.3.32

• NiceZyme view of 3.1.3.32

• EC2PDB: PDB structures for 3.1.3.32

• PRIAM entry for 3.1.3.32

• PUMA2 entry for 3.1.3.32

• IntEnz: Integrated Enzyme entry for 3.1.3.32

• MetaCyc entry for 3.1.3.32

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0046403: polynucleotide 3'-phosphatase

• AMIGO entry for GO code 0046403: polynucleotide 3'-phosphatase