Polyneuridine-aldehyde esterase

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In enzymology, a polyneuridine-aldehyde esterase (EC 3.1.1.78) is an enzyme that catalyzes the chemical reaction

polyneuridine aldehyde + H₂O $\rightleftharpoons$ 16-epivellosimine + CO₂ + methanol

Thus, the two substrates of this enzyme are polyneuridine aldehyde and H₂O, whereas its 3 products are 16-epivellosimine, CO₂, and methanol.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is polyneuridine aldehyde hydrolase (decarboxylating). Other names in common use include polyneuridine aldehyde esterase, and PNAE. This enzyme participates in indole and ipecac alkaloid biosynthesis.

[edit] References

IUBMB entry for 3.1.1.78
BRENDA references for 3.1.1.78 (Recommended.)
PubMed references for 3.1.1.78
PubMed Central references for 3.1.1.78
Google Scholar references for 3.1.1.78
Pfitzner A and Stockigt J (1983). "Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine ajmaline type alkaloids". Planta Med. 48: 221–227.
Pfitzner A and Stockigt J (1983). "Polyneuridine aldehyde esterase: an unusual specific enzyme involved in the biosynthesis of sarpagine type alkaloids". J. Chem. Soc. Chem. Commun.: 459–460.
Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J (2000). "The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family". Eur. J. Biochem. 267: 1397–406. PMID 10691977.
Mattern-Dogru E, Ma X, Hartmann J, Decker H, Stockigt J (2002). "Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis". Eur. J. Biochem. 269: 2889–96. PMID 12071952.