PNKP

From Wikipedia, the free encyclopedia

Polynucleotide kinase 3'-phosphatase

PDB rendering based on 2brf.

Available structures: 2brf

Identifiers

Symbol(s)

PNKP; PNK

External IDs

OMIM: 605610 MGI: 1891698 HomoloGene: 5247

Gene ontology
Molecular Function:	• damaged DNA binding • double-stranded DNA binding • endonuclease activity • ATP binding • kinase activity • transferase activity • hydrolase activity • purine nucleotide binding • nucleotide kinase activity • polynucleotide 3'-phosphatase activity • ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
Cellular Component:	• nucleus
Biological Process:	• nucleotide-excision repair, DNA damage removal • DNA-dependent DNA replication • DNA repair • response to oxidative stress • response to radiation • dephosphorylation • DNA damage response, detection of DNA damage • nucleotide phosphorylation

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_007254 (mRNA)
NP_009185 (protein)

NM_021549 (mRNA)
NP_067524 (protein)

Location

Chr 19: 55.06 - 55.06 Mb

Chr 7: 44.73 - 44.73 Mb

Pubmed search

[1]

[2]

Polynucleotide kinase 3'-phosphatase, also known as PNKP, is a human gene.^[1]

[edit] References

^ Entrez Gene: PNKP polynucleotide kinase 3'-phosphatase.

[edit] Further reading

Jilani A, Ramotar D, Slack C, et al. (1999). "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.". J. Biol. Chem. 274 (34): 24176–86. PMID 10446192.
Karimi-Busheri F, Daly G, Robins P, et al. (1999). "Molecular characterization of a human DNA kinase.". J. Biol. Chem. 274 (34): 24187–94. PMID 10446193.
Whitehouse CJ, Taylor RM, Thistlethwaite A, et al. (2001). "XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair.". Cell 104 (1): 107–17. PMID 11163244.
Chappell C, Hanakahi LA, Karimi-Busheri F, et al. (2002). "Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining.". EMBO J. 21 (11): 2827–32. doi:10.1093/emboj/21.11.2827. PMID 12032095.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Mani RS, Karimi-Busheri F, Fanta M, et al. (2004). "Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex.". Biochemistry 42 (41): 12077–84. doi:10.1021/bi030127b. PMID 14556639.
Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19.". Nature 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
Loizou JI, El-Khamisy SF, Zlatanou A, et al. (2004). "The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks.". Cell 117 (1): 17–28. PMID 15066279.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway.". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMID 15231748.
Wiederhold L, Leppard JB, Kedar P, et al. (2004). "AP endonuclease-independent DNA base excision repair in human cells.". Mol. Cell 15 (2): 209–20. doi:10.1016/j.molcel.2004.06.003. PMID 15260972.
Koch CA, Agyei R, Galicia S, et al. (2005). "Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV.". EMBO J. 23 (19): 3874–85. doi:10.1038/sj.emboj.7600375. PMID 15385968.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics.". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Audebert M, Salles B, Weinfeld M, Calsou P (2006). "Involvement of polynucleotide kinase in a poly(ADP-ribose) polymerase-1-dependent DNA double-strand breaks rejoining pathway.". J. Mol. Biol. 356 (2): 257–65. doi:10.1016/j.jmb.2005.11.028. PMID 16364363.
Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMID 17353931.
Karimi-Busheri F, Rasouli-Nia A, Allalunis-Turner J, Weinfeld M (2007). "Human polynucleotide kinase participates in repair of DNA double-strand breaks by nonhomologous end joining but not homologous recombination.". Cancer Res. 67 (14): 6619–25. doi:10.1158/0008-5472.CAN-07-0480. PMID 17638872.
Mani RS, Fanta M, Karimi-Busheri F, et al. (2007). "XRCC1 stimulates polynucleotide kinase by enhancing its damage discrimination and displacement from DNA repair intermediates.". J. Biol. Chem. 282 (38): 28004–13. doi:10.1074/jbc.M704867200. PMID 17650498.