PIGT

From Wikipedia, the free encyclopedia

Phosphatidylinositol glycan anchor biosynthesis, class T

Identifiers

PIGT; CGI-06; FLJ41596; MGC8909; NDAP

External IDs

OMIM: 610272 MGI: 1926178 HomoloGene: 6134

Gene ontology
Molecular Function:	• GPI-anchor transamidase activity • protein binding
Cellular Component:	• cytoplasm • endoplasmic reticulum • membrane • integral to membrane • cytoplasmic membrane-bound vesicle • GPI-anchor transamidase complex
Biological Process:	• attachment of GPI anchor to protein • neuron differentiation • neuron apoptosis

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_015937 (mRNA)
NP_057021 (protein)

NM_133779 (mRNA)
NP_598540 (protein)

Location

Chr 20: 43.48 - 43.49 Mb

Chr 2: 164.19 - 164.2 Mb

Pubmed search

[1]

[2]

Phosphatidylinositol glycan anchor biosynthesis, class T, also known as PIGT, is a human gene.^[1]

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.^[1]

[edit] Popular Culture

The PIGT gene was referenced in a sketch on the TV show Armstrong and Miller due to the habit of predictive text messaging phones to guess the relatively obscure 'PIGT' when the keys 7448 are pressed, instead of a common swear word.

[edit] References

^ ^a ^b Entrez Gene: PIGT phosphatidylinositol glycan anchor biosynthesis, class T.

[edit] Further reading

Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. (2003). "Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins.". Bioessays 25 (4): 367-85. doi:10.1002/bies.10254. PMID 12655644.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171-4. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149-56. PMID 9373149.
Lai CH, Chou CY, Ch'ang LY, et al. (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.". Genome Res. 10 (5): 703-13. PMID 10810093.
Ohishi K, Inoue N, Kinoshita T (2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.". EMBO J. 20 (15): 4088-98. doi:10.1093/emboj/20.15.4088. PMID 11483512.
Yu Y, Zhang C, Zhou G, et al. (2001). "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs.". Genome Res. 11 (8): 1392-403. doi:10.1101/gr.175501. PMID 11483580.
Fossey SC, Mychaleckyj JC, Pendleton JK, et al. (2001). "A high-resolution 6.0-megabase transcript map of the type 2 diabetes susceptibility region on human chromosome 20.". Genomics 76 (1-3): 45-57. doi:10.1006/geno.2001.6584. PMID 11549316.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20.". Nature 414 (6866): 865-71. doi:10.1038/414865a. PMID 11780052.
Vainauskas S, Maeda Y, Kurniawan H, et al. (2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.". J. Biol. Chem. 277 (34): 30535-42. doi:10.1074/jbc.M205402200. PMID 12052837.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T (2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.". J. Biol. Chem. 278 (16): 13959-67. doi:10.1074/jbc.M300586200. PMID 12582175.
Hong Y, Ohishi K, Kang JY, et al. (2004). "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.". Mol. Biol. Cell 14 (5): 1780-9. doi:10.1091/mbc.E02-12-0794. PMID 12802054.
Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.". Genome Res. 13 (10): 2265-70. doi:10.1101/gr.1293003. PMID 12975309.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.
Vainauskas S, Menon AK (2005). "Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.". J. Biol. Chem. 280 (16): 16402-9. doi:10.1074/jbc.M414253200. PMID 15713669.
Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.". DNA Res. 12 (2): 117-26. doi:10.1093/dnares/12.2.117. PMID 16303743.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.". Genome Res. 16 (1): 55-65. doi:10.1101/gr.4039406. PMID 16344560.
Li HL, Li Z, Qin LY, et al. (2006). "The novel neurotrophin-regulated neuronal development-associated protein, NDAP, mediates apoptosis.". FEBS Lett. 580 (7): 1723-8. doi:10.1016/j.febslet.2006.02.022. PMID 16516892.