PIGK

From Wikipedia, the free encyclopedia

Phosphatidylinositol glycan anchor biosynthesis, class K

Identifiers

PIGK; GPI8; MGC22559

External IDs

OMIM: 605087 MGI: 1913863 HomoloGene: 4002

Gene ontology
Molecular Function:	• legumain activity • GPI-anchor transamidase activity • protein binding • GPI anchor binding
Cellular Component:	• endoplasmic reticulum • membrane • integral to membrane • GPI-anchor transamidase complex
Biological Process:	• protein thiol-disulfide exchange • proteolysis • attachment of GPI anchor to protein

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

n/a

n/a

Refseq

NM_005482 (mRNA)
NP_005473 (protein)

XM_992234 (mRNA)
XP_997328 (protein)

Location

Chr 1: 77.33 - 77.46 Mb

n/a

Pubmed search

[1]

[2]

Phosphatidylinositol glycan anchor biosynthesis, class K, also known as PIGK, is a human gene.^[1]

This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.^[1]

[edit] References

^ ^a ^b Entrez Gene: PIGK phosphatidylinositol glycan anchor biosynthesis, class K.

[edit] Further reading

Benghezal M, Benachour A, Rusconi S, et al. (1997). "Yeast Gpi8p is essential for GPI anchor attachment onto proteins.". EMBO J. 15 (23): 6575–83. PMID 8978684.
Yu J, Nagarajan S, Knez JJ, et al. (1997). "The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase.". Proc. Natl. Acad. Sci. U.S.A. 94 (23): 12580–5. PMID 9356492.
Meyer U, Benghezal M, Imhof I, Conzelmann A (2000). "Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins.". Biochemistry 39 (12): 3461–71. PMID 10727241.
Ohishi K, Inoue N, Maeda Y, et al. (2000). "Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins.". Mol. Biol. Cell 11 (5): 1523–33. PMID 10793132.
Spurway TD, Dalley JA, High S, Bulleid NJ (2001). "Early events in glycosylphosphatidylinositol anchor addition. substrate proteins associate with the transamidase subunit gpi8p.". J. Biol. Chem. 276 (19): 15975–82. doi:10.1074/jbc.M010128200. PMID 11278620.
Ohishi K, Inoue N, Kinoshita T (2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.". EMBO J. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMID 11483512.
Vainauskas S, Maeda Y, Kurniawan H, et al. (2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.". J. Biol. Chem. 277 (34): 30535–42. doi:10.1074/jbc.M205402200. PMID 12052837.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T (2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.". J. Biol. Chem. 278 (16): 13959–67. doi:10.1074/jbc.M300586200. PMID 12582175.
Chen R, Anderson V, Hiroi Y, Medof ME (2003). "Proprotein interaction with the GPI transamidase.". J. Cell. Biochem. 88 (5): 1025–37. doi:10.1002/jcb.10439. PMID 12616539.
Hong Y, Ohishi K, Kang JY, et al. (2004). "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.". Mol. Biol. Cell 14 (5): 1780–9. doi:10.1091/mbc.E02-12-0794. PMID 12802054.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMID 16344560.
Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1.". Nature 441 (7091): 315–21. doi:10.1038/nature04727. PMID 16710414.