Talk:Phosphorylation
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How I can dephophorylate my protein sample? Please help (use alkaline Phosphatase or under strong alkaline conditions (beta elemination)...it really depends what you want to dephosphorylate (serine/threonine or tyrosine
My very personal point of view to this article: This article has the wrong contents. The definition in the first line is really really wrong. Phosph in terms of oxidative ~, photo ~, or substrate level ~ has the saem importance as protein phosphorylation. If you read the literature of 1950-1990, phosphorylation had no relevance to proteins. There so many more things than just fancy cancer research protein phosphorylation, Phosphoproteomics and Phosphorylomics! Cheers a Omics scientist
Does the phospphorylation sites have to reside in the cytosolic portion of the membrane proteins? Does secreted protein every get phosphorylated? I guess my question is: Are there kinases and phosphatases in the extracellular space? If someone can answer this question, could you please add it to the text. Thanks.
