Phospho-N-acetylmuramoyl-pentapeptide-transferase

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In enzymology, a phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) is an enzyme that catalyzes the chemical reaction

UDP-Mur₂Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate $\rightleftharpoons$ UMP + Mur₂Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)- diphosphoundecaprenol

Thus, the two substrates of this enzyme are UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) and undecaprenyl phosphate, whereas its 3 products are UMP, Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-, and diphosphoundecaprenol.

This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is UDP-MurAc(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala):undecaprenyl-phos phate phospho-N-acetylmuramoyl-pentapeptide-transferase. Other names in common use include MraY transferase, UDP-MurNAc-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala:C55-isoprenoid, alcohol transferase, UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla:undecaprenylphosphate, transferase, phospho-N-acetylmuramoyl pentapeptide translocase, phospho-MurNAc-pentapeptide transferase, phospho-NAc-muramoyl-pentapeptide translocase (UMP), phosphoacetylmuramoylpentapeptide translocase, and phosphoacetylmuramoylpentapeptidetransferase. This enzyme participates in peptidoglycan biosynthesis.

[edit] References

IUBMB entry for 2.7.8.13
BRENDA references for 2.7.8.13 (Recommended.)
PubMed references for 2.7.8.13
PubMed Central references for 2.7.8.13
Google Scholar references for 2.7.8.13
Heydanek MG Jr, Neuhaus FC (1969). "The initial stage in peptidoglycan synthesis. IV. Solubilization of phospho-N-acetylmuramyl-pentapeptide translocase". Biochemistry. 8: 1474–81. doi:10.1021/bi00832a024. PMID 5805290.
Higashi Y, Strominger JL, Sweeley CC (1967). "Structure of a lipid intermediate in cell wall peptidoglycan synthesis: a derivative of a C55 isoprenoid alcohol". Proc. Natl. Acad. Sci. U. S. A. 57: 1878–84. doi:10.1073/pnas.57.6.1878. PMID 5231417.
Struve WG, Sinha RK, Neuhaus FC (1966). "On the initial stage in peptidoglycan synthesis Phospho-N-acetylmuramyl-pentapeptide translocase (uridine monophosphate)". Biochemistry. 5: 82–93. doi:10.1021/bi00865a012. PMID 5938956.
van Heijenoort J (2001). "Recent advances in the formation of the bacterial peptidoglycan monomer unit". Nat. Prod. Rep. 18: 503–19. doi:10.1039/a804532a. PMID 11699883.