Pectin lyase

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In enzymology, a pectin lyase (EC 4.2.2.10) is an enzyme that catalyzes the chemical reaction

Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is (1->4)-6-O-methyl-alpha-D-galacturonan lyase. Other names in common use include pectin trans-eliminase, endo-pectin lyase, polymethylgalacturonic transeliminase, pectin methyltranseliminase, pectolyase, PL, PNL, and PMGL.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1IDJ, 1IDK, and 1QCX.

[edit] References

• IUBMB entry for 4.2.2.10
• BRENDA references for 4.2.2.10 (Recommended.)
• PubMed references for 4.2.2.10
• PubMed Central references for 4.2.2.10
• Google Scholar references for 4.2.2.10
• Albersheim P, Neukom H and Deuel H (1960). "Uber die Bildung von ungesattigten Abbauprodukten durch ein pekinabbauendes Enzym". Helv. Chim. Acta 43: 1422–1426. doi:10.1002/hlca.19600430525. 
• Pickersgill R, Jenkins J (1997). "Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases". Structure. 5: 677–89. doi:10.1016/S0969-2126(97)00222-0. PMID 9195887. 
• Kester HCM and Visser J (1994). "Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger". FEMS Microbiol. Lett. 120: 63–68. doi:10.1111/j.1574-6968.1994.tb07008.x. 
• Mutenda KE, Korner R, Christensen TM, Mikkelsen J, Roepstorff P (2002). "Application of mass spectrometry to determine the activity and specificity of pectin lyase A". Carbohydr. Res. 337: 1217–27. doi:10.1016/S0008-6215(02)00127-1. PMID 12110197. 

[edit] External links

The CAS registry number for this enzyme class is 9033-35-6.

• IUBMB entry for 4.2.2.10

• KEGG entry for 4.2.2.10

• BRENDA entry for 4.2.2.10

• NiceZyme view of 4.2.2.10

• EC2PDB: PDB structures for 4.2.2.10

• PRIAM entry for 4.2.2.10

• PUMA2 entry for 4.2.2.10

• IntEnz: Integrated Enzyme entry for 4.2.2.10

• MetaCyc entry for 4.2.2.10

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0047490: pectin lyase

• AMIGO entry for GO code 0047490: pectin lyase