PCBP2

From Wikipedia, the free encyclopedia

Poly(rC) binding protein 2

PDB rendering based on 1ztg.

Available structures: 1ztg, 2axy

Identifiers

Symbol(s)

PCBP2; HNRPE2; MGC110998; hnRNP-E2

External IDs

OMIM: 601210 MGI: 108202 HomoloGene: 74536

Gene ontology
Molecular Function:	• DNA binding • RNA binding • protein binding
Cellular Component:	• nucleus • cytoplasm • ribonucleoprotein complex
Biological Process:	• mRNA metabolic process

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_005016 (mRNA)
NP_005007 (protein)

NM_011042 (mRNA)
NP_035172 (protein)

Location

Chr 12: 52.13 - 52.16 Mb

Chr 15: 102.3 - 102.33 Mb

Pubmed search

[1]

[2]

Poly(rC) binding protein 2, also known as PCBP2, is a human gene.

The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene.^[1]

[edit] References

^ Entrez Gene: PCBP2 poly(rC) binding protein 2.

[edit] Further reading

Kiledjian M, Wang X, Liebhaber SA (1995). "Identification of two KH domain proteins in the alpha-globin mRNP stability complex.". EMBO J. 14 (17): 4357–64. PMID 7556077.
Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.". Eur. J. Biochem. 230 (2): 447–53. PMID 7607214.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
Tommerup N, Leffers H (1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A.". Genomics 32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID 8833161.
Blyn LB, Swiderek KM, Richards O, et al. (1996). "Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry.". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 11115–20. PMID 8855318.
Funke B, Zuleger B, Benavente R, et al. (1996). "The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins.". Nucleic Acids Res. 24 (19): 3821–8. PMID 8871564.
Blyn LB, Towner JS, Semler BL, Ehrenfeld E (1997). "Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA.". J. Virol. 71 (8): 6243–6. PMID 9223526.
Kiledjian M, DeMaria CT, Brewer G, Novick K (1997). "Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex.". Mol. Cell. Biol. 17 (8): 4870–6. PMID 9234743.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. PMID 9373149.
Czyzyk-Krzeska MF, Bendixen AC (1999). "Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA.". Blood 93 (6): 2111–20. PMID 10068686.
Wang Z, Day N, Trifillis P, Kiledjian M (1999). "An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro.". Mol. Cell. Biol. 19 (7): 4552–60. PMID 10373504.
Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog.". J. Biol. Chem. 274 (35): 24849–57. PMID 10455157.
Kim JH, Hahm B, Kim YK, et al. (2000). "Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.". J. Mol. Biol. 298 (3): 395–405. doi:10.1006/jmbi.2000.3687. PMID 10772858.
Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins.". Genomics 67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID 10936052.
Chang CJ, Luh HW, Wang SH, et al. (2002). "The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein.". DNA Cell Biol. 20 (9): 569–77. doi:10.1089/104454901317094981. PMID 11747608.
Yeap BB, Voon DC, Vivian JP, et al. (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.". J. Biol. Chem. 277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID 12011088.
Walter BL, Parsley TB, Ehrenfeld E, Semler BL (2002). "Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication.". J. Virol. 76 (23): 12008–22. PMID 12414943.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Abraham RS, Geyer SM, Price-Troska TL, et al. (2003). "Immunoglobulin light chain variable (V) region genes influence clinical presentation and outcome in light chain-associated amyloidosis (AL).". Blood 101 (10): 3801–8. doi:10.1182/blood-2002-09-2707. PMID 12515719.
Pickering BM, Mitchell SA, Evans JR, Willis AE (2003). "Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo.". Nucleic Acids Res. 31 (2): 639–46. PMID 12527772.