para-Nitrophenylphosphate
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Para-Nitrophenylphosphate | |
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IUPAC name | (4-nitrophenyl) dihydrogen phosphate |
Other names | pNPP |
Identifiers | |
CAS number | [33013-2] |
PubChem | |
SMILES | C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O |
Properties | |
Molecular formula | C6H6NO6P |
Molar mass | 219.088701 |
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. Under their influence the decay to yellow para-nitrophenol is catalysed. This product can be measured with a 405 nm spectrophotometer.