PARN

From Wikipedia, the free encyclopedia

Poly(A)-specific ribonuclease (deadenylation nuclease)

PDB rendering based on 1whv.

Available structures: 1whv, 2a1r, 2a1s

Identifiers

Symbol(s)

PARN; DAN

External IDs

OMIM: 604212 MGI: 1921358 HomoloGene: 31098

Gene ontology
Molecular Function:	• nucleotide binding • magnesium ion binding • RNA binding • mRNA 3'-UTR binding • exonuclease activity • poly(A)-specific ribonuclease activity • protein binding • hydrolase activity
Cellular Component:	• nucleus • cytoplasm
Biological Process:	• mRNA catabolic process, nonsense-mediated decay • female gamete generation • RNA modification

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_002582 (mRNA)
NP_002573 (protein)

NM_028761 (mRNA)
NP_083037 (protein)

Location

Chr 16: 14.44 - 14.63 Mb

Chr 16: 13.45 - 13.58 Mb

Pubmed search

[1]

[2]

Poly(A)-specific ribonuclease (deadenylation nuclease), also known as PARN, is a human gene.^[1]

Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. The amino acid sequence of poly(A)-specific ribonuclease shows homology to the RNase D family of 3'-exonucleases. The protein appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits.^[1]

[edit] References

^ ^a ^b Entrez Gene: PARN poly(A)-specific ribonuclease (deadenylation nuclease).

[edit] Further reading

Körner CG, Wahle E (1997). "Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease.". J. Biol. Chem. 272 (16): 10448–56. PMID 9099687.
Körner CG, Wormington M, Muckenthaler M, et al. (1998). "The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.". EMBO J. 17 (18): 5427–37. doi:10.1093/emboj/17.18.5427. PMID 9736620.
Buiting K, Körner C, Ulrich B, et al. (2000). "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11-->q13.". Cytogenet. Cell Genet. 87 (1-2): 125–31. PMID 10640832.
Dehlin E, Wormington M, Körner CG, Wahle E (2000). "Cap-dependent deadenylation of mRNA.". EMBO J. 19 (5): 1079–86. doi:10.1093/emboj/19.5.1079. PMID 10698948.
Martinez J, Ren YG, Thuresson AC, et al. (2000). "A 54-kDa fragment of the Poly(A)-specific ribonuclease is an oligomeric, processive, and cap-interacting Poly(A)-specific 3' exonuclease.". J. Biol. Chem. 275 (31): 24222–30. doi:10.1074/jbc.M001705200. PMID 10801819.
Gao M, Fritz DT, Ford LP, Wilusz J (2000). "Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro.". Mol. Cell 5 (3): 479–88. PMID 10882133.
Gao M, Wilusz CJ, Peltz SW, Wilusz J (2001). "A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements.". EMBO J. 20 (5): 1134–43. doi:10.1093/emboj/20.5.1134. PMID 11230136.
Martînez J, Ren YG, Nilsson P, et al. (2001). "The mRNA cap structure stimulates rate of poly(A) removal and amplifies processivity of degradation.". J. Biol. Chem. 276 (30): 27923–9. doi:10.1074/jbc.M102270200. PMID 11359775.
Copeland PR, Wormington M (2001). "The mechanism and regulation of deadenylation: identification and characterization of Xenopus PARN.". RNA 7 (6): 875–86. PMID 11424938.
Chen CY, Gherzi R, Ong SE, et al. (2002). "AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.". Cell 107 (4): 451–64. PMID 11719186.
Ren YG, Martínez J, Virtanen A (2002). "Identification of the active site of poly(A)-specific ribonuclease by site-directed mutagenesis and Fe(2+)-mediated cleavage.". J. Biol. Chem. 277 (8): 5982–7. doi:10.1074/jbc.M111515200. PMID 11742007.
Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus.". Curr. Biol. 12 (1): 1–11. PMID 11790298.
Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus.". Mol. Biol. Cell 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMID 12429849.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Lai WS, Kennington EA, Blackshear PJ (2003). "Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease.". Mol. Cell. Biol. 23 (11): 3798–812. PMID 12748283.
Lejeune F, Li X, Maquat LE (2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities.". Mol. Cell 12 (3): 675–87. PMID 14527413.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gherzi R, Lee KY, Briata P, et al. (2004). "A KH domain RNA binding protein, KSRP, promotes ARE-directed mRNA turnover by recruiting the degradation machinery.". Mol. Cell 14 (5): 571–83. doi:10.1016/j.molcel.2004.05.002. PMID 15175153.
Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation.". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMID 15231747.
Ren YG, Kirsebom LA, Virtanen A (2005). "Coordination of divalent metal ions in the active site of poly(A)-specific ribonuclease.". J. Biol. Chem. 279 (47): 48702–6. doi:10.1074/jbc.M403858200. PMID 15358788.