OxyS RNA
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OxyS RNA is a small non-coding RNA which is induced in response to oxidative stress in Escherichia coli. This RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, by an antisense mechanism, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(s) subunit of RNA polymerase.[1] OxyS is bound by the Hfq protein, that increases the OxyS RNA interaction with its target messages.[2]. The 109 nucleotide RNA is thought to be composed of three stem-loops.
[edit] Target genes of OxyS
A number of additional targets were recently predicted and verified using microarray analysis.[3] These are listed below:
- yobF - confirmed by Northern analysis
- yaiZ
- rumA
- wrbA - confirmed by Northern analysis
- ybaY - confirmed by Northern analysis
[edit] References
- ^ Altuvia, S; Zhang A, Argaman L, Tiwari A, Storz G (1998). "The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding". EMBO J 17: 6069–6075. doi: . PMID 9774350.
- ^ Zhang, A; Wassarman KM, Ortega J, Steven AC, Storz G (2002). "The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs". Mol Cell 9: 11–22. doi: . PMID 11804582.
- ^ Tjaden B, Goodwin SS, Opdyke JA, et al (2006). "Target prediction for small, noncoding RNAs in bacteria". Nucleic Acids Res. 34 (9): 2791–802. doi: . PMID 16717284.