From Wikipedia, the free encyclopedia
| Nuclear RNase P |
 |
| Type: |
Gene; ribozyme; |
| 2° structure: |
Published; PubMed |
| Seed alignment: |
Brown JW, The Ribonuclease P Database, PubMed |
| Avg length: |
298.8 nucleotides |
| Avg identity: |
42% |
|
In molecular biology, nuclear ribonuclease P (RNase P) is a ubiquitous endoribonuclease, found in archaea, bacteria and eukarya as well as chloroplasts and mitochondria. Its best characterised enzyme activity is the generation of mature 5'-ends of tRNAs by cleaving the 5'-leader elements of precursor-tRNAs. Cellular RNase Ps are ribonucleoproteins. The RNA from bacterial RNase Ps retains its catalytic activity in the absence of the protein subunit, i.e. it is a ribozyme. Isolated eukaryotic and archaeal RNase P RNA has not been shown to retain its catalytic function, but is still essential for the catalytic activity of the holoenzyme. Although the archaeal and eukaryotic holoenzyme s have a much greater protein content than the bacterial ones, the RNA cores from all three lineages are homologous -- the helices corresponding to P1, P2, P3, P4, and P10/11 are common to all cellular RNase P RNAs. Yet there is considerable sequence variation, particularly among the eukaryotic RNAs.
[edit] References
- Frank, DN; Pace NR (1998). "Ribonuclease P: unity and diversity in a tRNA processing ribozyme". Annu Rev Biochem 67: 153–180. doi:10.1146/annurev.biochem.67.1.153. PMID 9759486.
- Brown, JW (1999). "The Ribonuclease P Database". Nucleic Acids Res 27: 314–. doi:10.1093/nar/27.1.314. PMID 9847214.
- Frank, DN; Adamidi C, Ehringer MA, Pitulle C, Pace NR (2000). "Phylogenetic-comparative analysis of the eukaryal ribonuclease P RNA". RNA 6: 1895–1904. doi:10.1017/S1355838200001461. PMID 11142387.
- Xiao, S; Scott F, Fierke CA, Engelke DR (2002). "Eukaryotic Ribonuclease P: A Plurality of Ribonucleoprotein Enzymes". Annu Rev Biochem 71: 165–189. doi:10.1146/annurev.biochem.71.110601.135352. PMID 12045094.
- Marquez, SM; Harris JK, Kelley ST, Brown JW, Dawson SC, Roberts EC, Pace NR (2005). "Structural implications of novel diversity in eucaryal RNase P RNA". RNA. 11: 739–751. doi:10.1261/rna.7211705. PMID 15811915.
[edit] External links
