NSP3 (rotavirus)

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Non Structural Rotavirus Protein 3
Identifiers
Symbol NSP3
PDB 1LJ2
Other data

Rotavirus protein NSP3 (NS34) is bound to the 3' end consensus sequence of viral mRNAs in infected cells.[1]

Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3: using short oligoribonucleotides, it was established that the minimal RNA sequence required for binding of NSP3A is GACC.[2]


Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F. And NSP3A, by taking the place of PABP on eIF4GI, is responsible for the shut-off of cellular protein synthesis.[3]

Expression of NSP3 in mammalian cells allows the efficient translation of virus-like mRNA: NSP3 forms a link between viral mRNA and the cellular translation machinery and hence is a functional analogue of cellular poly(A)-binding protein.[4] Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.[5]

Cellular vs Rotavirus Translation
Cellular vs Rotavirus Translation

Using the yeast two-hybrid assay, RoXan a novel cellular protein was found to bind NSP3. The interaction between NSP3 and RoXaN does not impair the interaction between NSP3 and eIF4GI, and a ternary complex made of NSP3, RoXaN, and eIF4G I can be detected in rotavirus-infected cells, implicating RoXaN in translation regulation.[6]


[edit] References

  1. ^ Poncet, D; C Aponte, J Cohen (1993- Jun). "Rotavirus protein NSP3 (NS34) is bound to the 3' end consensus sequence of viral mRNAs in infected cells.". Journal of virology 67 (6): 3159-65. 8388495. 
  2. ^ Poncet, D; S Laurent, J Cohen (1994- Sep- 1). "Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3.". The EMBO journal 13 (17): 4165-73. 8076612. 
  3. ^ Piron, M; P Vende, J Cohen, D Poncet (1998- Oct- 1). "Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F.". The EMBO journal 17 (19): 5811-21. 9755181 doi:10.1093/emboj/17.19.5811. 
  4. ^ Vende, P; M Piron, N Castagné, D Poncet (2000- Aug). "Efficient translation of rotavirus mRNA requires simultaneous interaction of NSP3 with the eukaryotic translation initiation factor eIF4G and the mRNA 3' end.". Journal of virology 74 (15): 7064-71. 10888646. 
  5. ^ Groft, Caroline M; Stephen K Burley (2002- Jun). "Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization.". Molecular cell 9 (6): 1273-83. 12086624. 
  6. ^ Vitour, Damien; Pierre Lindenbaum, Patrice Vende, Michelle M Becker, Didier Poncet (2004- Apr). "RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3.". Journal of virology 78 (8): 3851-62. 15047801. 


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