Nitrite reductase (NAD(P)H)

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In enzymology, a nitrite reductase [NAD(P)H] (EC 1.7.1.4) is an enzyme that catalyzes the chemical reaction

ammonium hydroxide + 3 NAD(P)+ + H₂O $\rightleftharpoons$ nitrite + 3 NAD(P)H + 3 H⁺

The 4 substrates of this enzyme are ammonium hydroxide, NAD⁺, NADP⁺, and H₂O, whereas its 4 products are nitrite, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is ammonium-hydroxide:NAD(P)+ oxidoreductase. Other names in common use include nitrite reductase (reduced nicotinamide adenine dinucleotide, (phosphate)), NADH-nitrite oxidoreductase, NADPH-nitrite reductase, assimilatory nitrite reductase, nitrite reductase [NAD(P)H2], and NAD(P)H2:nitrite oxidoreductase. This enzyme participates in nitrogen metabolism. It has 3 cofactors: FAD, Iron, and Siroheme.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2JO6.

[edit] References

IUBMB entry for 1.7.1.4
BRENDA references for 1.7.1.4 (Recommended.)
PubMed references for 1.7.1.4
PubMed Central references for 1.7.1.4
Google Scholar references for 1.7.1.4
Cammack R, Jackson RH, Cornish-Bowden A, Cole JA (1982). "Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12". Biochem. J. 207: 333–9. PMID 6297458.
Lazzarini RA and Atkinson DE (1961). "A triphosphopyridine nucleotide-specific nitrite reductase from Escherichia coli". J. Biol. Chem. 236: 3330–3335.
Nicholas DJD, Medina A and Jones OTG (1960). "A nitrite reductase from Neurospora crassa". Biochim. Biophys. Acta 37: 468. doi:10.1016/0006-3002(60)90503-5.
Taniguchi H, Mitsui H, Nakamura K and Egami F. "Ann. Acad. Sci. Fenn. Ser. A II 60 (1955) 200".
Colandene JD, Garrett RH (1996). "Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa". J. Biol. Chem. 271: 24096–104. doi:10.1074/jbc.271.39.24096. PMID 8798648.