Negative elongation factor

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NELF (Negative elongation factor) is a four subunit protein (NELF-A, NELF-B, NELF-C or NELF-D, NELF-E) that affects transcription by RNA polymerase II. Subunit NELF-E is also known as RDBP.[1][2] The NELF-A subunit is encoded by the gene WHSC2, Wolf-Hirschhorn syndrome candidate 2. NELF-C and NELF-D are probably peptides resulting from the same mRNA with different translation initiation site, possibly differing only in an extra 9 amino acids for NELF-C at the N-terminus. Or, it is possible they arise from different mRNAs. A single NELF complex consists of either NELF-C or NELF-D but not both. Microsequencing analysis showed that NELF-B was the protein previously identified as the protein encoded by the gene COBRA1, and shown to interact with BRCA1.[2]

[edit] References

  1. ^ Yamaguchi, Y.; Takagi, T.; Wada, T.; Yano, K.; Furuya, A.; Sugimoto, S.; Hasegawa, J.; Handa, H. (1999). "NELF, a Multisubunit Complex Containing RD, Cooperates with DSIF to Repress RNA Polymerase II Elongation". Cell 97: 41-51. PMID 10199401. 
  2. ^ a b Narita, Takashi; Yamaguchi, Yuki ; Yano, Keiichi; Sugimoto, Seiji; Chanarat, Sittinan; Wada, Tadashi; Kim, Dong-ki; Hasegawa, Jun; Omori, Masashi; Inukai, Naoto; Endoh, Masaki; Yamada, Tomoko; Handa, Hiroshi (2003). "Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex". MOLECULAR AND CELLULAR BIOLOGY 23: 1863–1873. doi:10.1128/MCB.23.6.1863-1873.2003. PMID 12612062. 
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