NAGPA

From Wikipedia, the free encyclopedia

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Identifiers

NAGPA; APAA; UCE

External IDs

OMIM: 607985 MGI: 1351598 HomoloGene: 8466

Gene ontology
Molecular Function:	• N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity • hydrolase activity
Cellular Component:	• membrane • integral to membrane
Biological Process:	• carbohydrate metabolic process • protein modification process • protein targeting to lysosome • lysosome organization and biogenesis

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_016256 (mRNA)
NP_057340 (protein)

NM_013796 (mRNA)
NP_038824 (protein)

Location

Chr 16: 5.01 - 5.02 Mb

Chr 16: 5.11 - 5.12 Mb

Pubmed search

[1]

[2]

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase, also known as NAGPA, is a human gene.^[1]

Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.^[1]

[edit] References

^ ^a ^b Entrez Gene: NAGPA N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase.

[edit] Further reading

Lee JK, Pierce M (1995). "Purification and characterization of human serum N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase.". Arch. Biochem. Biophys. 319 (2): 413-25. doi:10.1006/abbi.1995.1312. PMID 7786023.
Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio.1996.0138. PMID 8619474.
Page T, Zhao KW, Tao L, Miller AL (1997). "Purification and characterization of human lymphoblast N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase.". Glycobiology 6 (6): 619-26. PMID 8922957.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353-8. PMID 9110174.
Kornfeld R, Bao M, Brewer K, et al. (2000). "Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase.". J. Biol. Chem. 274 (46): 32778-85. PMID 10551838.
Rohrer J, Kornfeld R (2001). "Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network.". Mol. Biol. Cell 12 (6): 1623-31. PMID 11408573.
Lee WS, Rohrer J, Kornfeld R, Kornfeld S (2002). "Multiple signals regulate trafficking of the mannose 6-phosphate-uncovering enzyme.". J. Biol. Chem. 277 (5): 3544-51. doi:10.1074/jbc.M108531200. PMID 11723124.
Do H, Lee WS, Ghosh P, et al. (2002). "Human mannose 6-phosphate-uncovering enzyme is synthesized as a proenzyme that is activated by the endoprotease furin.". J. Biol. Chem. 277 (33): 29737-44. doi:10.1074/jbc.M202369200. PMID 12058031.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.
Nair P, Schaub BE, Huang K, et al. (2005). "Characterization of the TGN exit signal of the human mannose 6-phosphate uncovering enzyme.". J. Cell. Sci. 118 (Pt 13): 2949-56. doi:10.1242/jcs.02434. PMID 15976452.