NAD+ nucleosidase

From Wikipedia, the free encyclopedia

In enzymology, a NAD+ nucleosidase (EC 3.2.2.5) is an enzyme that catalyzes the chemical reaction

NAD⁺ + H₂O ADP-ribose + nicotinamide

Thus, the two substrates of this enzyme are NAD⁺ and H₂O, whereas its two products are ADP-ribose and nicotinamide.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is NAD+ glycohydrolase. Other names in common use include NADase, DPNase, DPN hydrolase, NAD hydrolase, diphosphopyridine nucleosidase, nicotinamide adenine dinucleotide nucleosidase, NAD glycohydrolase, NAD nucleosidase, and nicotinamide adenine dinucleotide glycohydrolase. This enzyme participates in nicotinate and nicotinamide metabolism and calcium signaling pathway.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 25 structures have been solved for this class of enzymes, with PDB accession codes 1ISF, 1ISG, 1ISH, 1ISI, 1ISJ, 1ISM, 1LBE, 1R0S, 1R12, 1R15, 1R16, 1YH3, 1ZVM, 2EF1, 2HCT, 2I65, 2I66, 2I67, 2O3Q, 2O3R, 2O3S, 2O3T, 2O3U, 2PGJ, and 2PGL.

[edit] References

• IUBMB entry for 3.2.2.5
• BRENDA references for 3.2.2.5 (Recommended.)
• PubMed references for 3.2.2.5
• PubMed Central references for 3.2.2.5
• Google Scholar references for 3.2.2.5
• HOFMANN EC, RAPOPORT S (1955). "[DPN- and TPN-specific nucleosidases in erythrocytes.]". Biochim. Biophys. Acta. 18: 296. PMID 13276383. 
• Nakazawa K, Ueda K, Honjo T, Yoshihara K, Nishizuka Y, Hayaishi O (1968). "Nicotinamide adenine dinucleotide glycohydrolases and poly adenosine diphosphate ribose synthesis in rat liver". Biochem. Biophys. Res. Commun. 32: 143–9. PMID 5672131. 
• Ueda K, Fukushima M, Okayama H, Hayaishi O (1975). "Nicotinamide adenine dinucleotide glycohydrolase from rat liver nuclei. Isolation and characterization of a new enzyme". J. Biol. Chem. 250: 7541–6. PMID 240831. 
• Morikawa K (2002). "Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities". J. Mol. Biol. 316: 711–23. PMID 11866528. 

[edit] External links

The CAS registry number for this enzyme class is 9032-65-9.

• IUBMB entry for 3.2.2.5

• KEGG entry for 3.2.2.5

• BRENDA entry for 3.2.2.5

• NiceZyme view of 3.2.2.5

• EC2PDB: PDB structures for 3.2.2.5

• PRIAM entry for 3.2.2.5

• PUMA2 entry for 3.2.2.5

• IntEnz: Integrated Enzyme entry for 3.2.2.5

• MetaCyc entry for 3.2.2.5

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0003953: NAD+ nucleosidase

• AMIGO entry for GO code 0003953: NAD+ nucleosidase