N-hydroxythioamide S-beta-glucosyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a N-hydroxythioamide S-beta-glucosyltransferase (EC 2.4.1.195) is an enzyme that catalyzes the chemical reaction

UDP-glucose + N-hydroxy-2-phenylethanethioamide $\rightleftharpoons$ UDP + desulfoglucotropeolin

Thus, the two substrates of this enzyme are UDP-glucose and N-hydroxy-2-phenylethanethioamide, whereas its two products are UDP and desulfoglucotropeolin.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:N-hydroxy-2-phenylethanethioamide S-beta-D-glucosyltransferase. Other names in common use include desulfoglucosinolate-uridine diphosphate glucosyltransferase, uridine diphosphoglucose-thiohydroximate glucosyltransferase, thiohydroximate beta-D-glucosyltransferase, UDPG:thiohydroximate glucosyltransferase, thiohydroximate S-glucosyltransferase, thiohydroximate glucosyltransferase, and UDP-glucose:thiohydroximate S-beta-D-glucosyltransferase.

[edit] References

IUBMB entry for 2.4.1.195
BRENDA references for 2.4.1.195 (Recommended.)
PubMed references for 2.4.1.195
PubMed Central references for 2.4.1.195
Google Scholar references for 2.4.1.195
Jain JC, Reed DW, GrootWassink JW, Underhill EW (1989). "A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species". Anal. Biochem. 178: 137–40. doi:10.1016/0003-2697(89)90369-2. PMID 2524977.
Reed DW, Davin L, Jain JC, Deluca V, Nelson L, Underhill EW (1993). "Purification and properties of UDP-glucose:thiohydroximate glucosyltransferase from Brassica napus L. seedlings". Arch. Biochem. Biophys. 305: 526–32. doi:10.1006/abbi.1993.1456. PMID 8373190.
Fahey JW, Zalcmann AT, Talalay P (2001). "The chemical diversity and distribution of glucosinolates and isothiocyanates among plants". Phytochemistry. 56: 5–51. doi:10.1016/S0031-9422(00)00316-2. PMID 11198818.
TF and Abel S (2004). "Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis". Plant J. 40: 893–908. doi:10.1111/j.1365-313X.2004.02261.x.