N-acyl-D-amino-acid deacylase

From Wikipedia, the free encyclopedia

In enzymology, a N-acyl-D-amino-acid deacylase (EC 3.5.1.81) is an enzyme that catalyzes the chemical reaction

N-acyl-D-amino acid + H₂O $\rightleftharpoons$ an acid + D-amino acid

Thus, the two substrates of this enzyme are N-acyl-D-amino acid and H₂O, whereas its two products are acid and D-amino acid.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-amino acid amidohydrolase. It employs one cofactor, zinc.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1M7J, 1RJP, 1RJQ, 1RJR, 1RK5, 1RK6, 1V4Y, and 1V51.

[edit] References

IUBMB entry for 3.5.1.81
BRENDA references for 3.5.1.81 (Recommended.)
PubMed references for 3.5.1.81
PubMed Central references for 3.5.1.81
Google Scholar references for 3.5.1.81
Wakayama M, Katsuno Y, Hayashi S, Miyamoto Y, Sakai K, Moriguchi M (1995). "Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli". Biosci. Biotechnol. Biochem. 59: 2115–9. PMID 8541651.
Wakayama M, Hayashi S, Yatsuda Y, Katsuno Y, Sakai K, Moriguchi M (1996). "Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification". Protein. Expr. Purif. 7: 395–9. PMID 8776758.