N-acetylglucosaminylphosphatidylinositol deacetylase

From Wikipedia, the free encyclopedia

In enzymology, a N-acetylglucosaminylphosphatidylinositol deacetylase (EC 3.5.1.89) is an enzyme that catalyzes the chemical reaction

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H₂O 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate

Thus, the two substrates of this enzyme are 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol and H₂O, whereas its two products are 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol and acetate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase. Other names in common use include N-acetyl-D-glucosaminylphosphatidylinositol acetylhydrolase, N-acetylglucosaminylphosphatidylinositol de-N-acetylase, GlcNAc-PI de-N-acetylase, GlcNAc-PI deacetylase, and acetylglucosaminylphosphatidylinositol deacetylase. This enzyme participates in 3 metabolic pathways: glycosylphosphatidylinositol(gpi)-anchor, [[]], and glycan structures - biosynthesis 2.

[edit] References

• IUBMB entry for 3.5.1.89
• BRENDA references for 3.5.1.89 (Recommended.)
• PubMed references for 3.5.1.89
• PubMed Central references for 3.5.1.89
• Google Scholar references for 3.5.1.89
• Doering TL, Masterson WJ, Englund PT, Hart GW (1989). "Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine". J. Biol. Chem. 264: 11168–73. PMID 2525555. 
• Kinoshita T (1997). "Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase". J. Biol. Chem. 272: 15834–40. PMID 9188481. 
• Watanabe R, Ohishi K, Maeda Y, Nakamura N, Kinoshita T (Pt 1). "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis". Biochem. J. 339: 185–92. PMID 10085243. 
• JS, Ferguson MA (2001). "Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors". EMBO. J. 20: 3322–32. PMID 11432820. 

[edit] External links

• IUBMB entry for 3.5.1.89

• KEGG entry for 3.5.1.89

• BRENDA entry for 3.5.1.89

• NiceZyme view of 3.5.1.89

• EC2PDB: PDB structures for 3.5.1.89

• PRIAM entry for 3.5.1.89

• PUMA2 entry for 3.5.1.89

• IntEnz: Integrated Enzyme entry for 3.5.1.89

• MetaCyc entry for 3.5.1.89

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0000225: N-acetylglucosaminylphosphatidylinositol deacetylase

• AMIGO entry for GO code 0000225: N-acetylglucosaminylphosphatidylinositol deacetylase